Preparation process of high-purity NMN based on enzymatic method

Abstract

The invention relates to the technical field of NMN preparation and discloses a preparation process of high-purity NMN based on an enzymatic method. The preparation process comprises the following steps: (1) raw material pretreatment: performing microfiltration and nanofiltration on nicotinamide ribose at first, and collecting concentrated crude product solution; (2) a production process: enablingthe crude product solution to react under the catalysis of nicotinamide ribokinase or recombinant cells containing the nicotinamide ribokinase to generate beta-nicotinamide mononucleotide; and (3) apurification process: adjusting the pH value of the produced beta-nicotinamide mononucleotide solution to 3-7, sampling the beta-nicotinamide mononucleotide solution on a reversed-phase high-performance liquid chromatography preparation column, and carrying out gradient elution and purification to obtain the purified beta-nicotinamide mononucleotide solution. The preparation process of the high-purity NMN based on the enzyme method ensures the productivity of the nicotinamide mononucleotide, improves the purity of the product, and can make the nicotinamide mononucleotide more obvious in the effect of relieving and improving the injury of ischemic heart and brain tissues. The nicotinamide mononucleotide can better improve oxidation-related degenerative diseases and body dysfunction.

Description

technical field [0001] The invention relates to the technical field of NMN preparation, in particular to an enzyme-based high-purity NMN preparation process. Background technique [0002] NMN (nicotinamide mononucleotide) is the product of nicotinamide phosphoribosyltransferase reaction and is one of the key precursors of NAD+. In mammals, NMN is generated from nicotinamide under the catalysis of Nampt, and then NMN is catalyzed by nicotinamide mononucleotide adenylyltransferase to generate NAD+. Extracellular NMN needs to be dephosphorylated and transformed into nicotinamide riboside to enter the liver cells. After entering the cell, NR is phosphorylated by nicotinamide riboside kinase 1 to generate NMN, and then NMN combines with ATP to generate NAD+. NMN exerts its physiological functions by converting into NAD+ in the human body, such as activating NAD+ substrate-dependent enzyme Sirt1 (histone deacetylase, also known as sirtuin), regulating cell survival and death, mai...

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Problems solved by technology

[0004] The traditional production method of nicotinamide mononucleotide is to use nicotinamide ribose as raw material and phosphorylate it with phosphorus oxychloride, which has low productivity, low product purity, and uses a large amount of organic solvents, which seriously damages the environment. The effect of nicotinamide mononucleotide on alleviating and improving ischemic heart and brain tissue damage is not obvious, and nicotinamide mononucleotide cannot improve oxidation-related degenerative diseases and physical dysfunction to a large extent. Therefore, we propose a A kind of high-purity NMN preparation process based on enzymatic method