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Novel specific long-acting bacterial attractant for lucilia sericata and preparation method thereof

A mercerized green fly and attractant technology, which is applied in the fields of attracting pests, botanical equipment and methods, biochemical equipment and methods, etc. Long-lasting effect with simple preparation method

Inactive Publication Date: 2020-07-28
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the continuous promotion of chemical control, the abuse and irrational use of insecticides have caused Lucilia sericata to develop resistance to many kinds of insecticides, resulting in the viciousness of "the more you kill, the harder it is to kill". At the same time, the unreasonable use of pesticides has brought many negative effects, such as food safety (pesticide residues exceed the standard), killing natural enemies and human and animal poisoning incidents, etc.

Method used

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  • Novel specific long-acting bacterial attractant for lucilia sericata and preparation method thereof
  • Novel specific long-acting bacterial attractant for lucilia sericata and preparation method thereof
  • Novel specific long-acting bacterial attractant for lucilia sericata and preparation method thereof

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preparation example Construction

[0038] The embodiment of the present invention also discloses a preparation method of a novel Lucilia sericata-specific long-acting bacterial attractant, which specifically includes the following steps:

[0039] (1) Preparation of bacterial cells: add bacteria isolated from the intestine of Lucilia sericata to LB medium, then shake the bacteria to the logarithmic phase at 37°C and 100 rpm; take out the medium and add it to a 10ml centrifuge tube, Centrifuge for 10 minutes, and collect the sediment as bacterial cells;

[0040] (2) According to a kind of novel Lucilia sericata-specific long-acting bacterial attractant as mentioned above, it is standby to take raw materials;

[0041] (3) Prepare bacterial growth matrix: mix soybean meal, fish meal, agar powder, potassium chloride, glycerin, urea and water evenly, then carry out autoclaving, then cool to 55°C to make bacterial growth matrix;

[0042] (4) Prepare Lucilia sericata attractant: add the bacterial cells cultivated over...

Embodiment 1

[0045]Embodiment 1 of the present invention discloses a novel Lucilia sericata-specific long-acting bacterial attractant, including: 5g Lucilia sericata intestinal bacterial thallus, 1g soybean powder, 1g fish meal, 1.0g agar powder, 0.5g chlorine dioxide in 100g Potassium chloride, 1g glycerin, 0.1g urea and the rest of water.

[0046] Embodiment 1 of the present invention also discloses a preparation method of a novel Lucilia sericata-specific long-acting bacterial attractant, specifically comprising the following steps:

[0047] (1) Preparation of bacterial cells: add Klebsiella oxytoca isolated from the intestine of Lucilia sericata to LB medium, then shake the bacteria to the logarithmic phase at 37°C and 100 rpm; take out the medium and add Put it into a 10ml centrifuge tube, centrifuge for 10 minutes, and collect the sediment as bacterial cells;

[0048] (2) According to a kind of novel Lucilia sericata-specific long-acting bacterial attractant as mentioned above, it i...

Embodiment 2

[0052] Embodiment 2 of the present invention discloses a novel Lucilia sericata-specific long-acting bacterial attractant, including components in 100g: 10g Lucilia sericata intestinal bacterial thallus, 5g soybean meal, 5g fish meal, 1.5g agar powder, 1g Potassium chloride, 3g glycerin, 0.5g urea and the rest of water.

[0053] Embodiment 2 of the present invention also discloses a preparation method of a novel Lucilia sericata-specific long-acting bacterial attractant, specifically comprising the following steps:

[0054] (1) Preparation of bacterial cells: add bacteria isolated from the intestine of Lucilia sericata to LB medium, then shake the bacteria to the logarithmic phase at 37°C and 100 rpm; take out the medium and add it to a 10ml centrifuge tube, Centrifuge for 10 minutes, and collect the sediment as bacterial cells;

[0055] (2) According to a kind of novel Lucilia sericata-specific long-acting bacterial attractant as mentioned above, it is standby to take raw ma...

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Abstract

The invention discloses a novel specific long-acting bacterial attractant for lucilia sericata. The attractant is prepared from the following components in parts by weight (100 parts by weight): 5-10parts of enterobacteria thalli of lucilia sericata, 1-5 parts of soybean meal, 1-5 parts of fish meal, 1.0-1.5 parts of agar powder, 0.5-1 part of potassium chloride, 1-3 parts of glycerinum, 0.1-0.5part of urea and the balance of water. The invention further provides a preparation method of the novel specific long-acting bacterial attractant for lucilia sericata. According to the invention, thepreparation method is simple, the raw material cost is low, the prepared attractant has characteristics of long-term effect and insensitivity to rainfall, wind blowing and other adverse weather conditions, and the specific attractant for lucilia sericata can be used for density monitoring of lucilia sericata, can be used for trapping and killing lucilia sericata, and has good function expansibility.

Description

technical field [0001] The invention relates to the technical field of fly control, in particular to a novel Lucilia sericata-specific long-acting bacterial attractant and a preparation method thereof. Background technique [0002] Lucilia sericata (Lucilia sericata) belongs to the genus Calligraphy of the Calliphoridae subfamily Calliphora of Diptera. It is a kind of medical vector insect distributed in the world. It can mechanically transmit a variety of pathogenic microorganisms and cause infectious diseases. The larvae are necrophagous , can lay eggs in human rotten wounds, boils, or parasitize in human digestive tract, wounds, facial features and other parts, causing human digestive tract myiasis, nasal myiasis, etc., threatening human health; Lucilia sericata can also It causes myiasis in livestock and poultry, which is harmful to animal husbandry. [0003] At the same time, Lucilia sericata is also an important pest of ham and salted carp products. During the proces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/20A01P19/00A01P7/04C12N1/20C12R1/22
CPCA01N25/006C12N1/20C12N1/205C12R2001/22
Inventor 张忠张瑞玲万晴乐倩倩黄振东
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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