Novel specific long-acting bacterial attractant for lucilia sericata and preparation method thereof
A mercerized green fly and attractant technology, which is applied in the fields of attracting pests, botanical equipment and methods, biochemical equipment and methods, etc. Long-lasting effect with simple preparation method
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[0038] The embodiment of the present invention also discloses a preparation method of a novel Lucilia sericata-specific long-acting bacterial attractant, which specifically includes the following steps:
[0039] (1) Preparation of bacterial cells: add bacteria isolated from the intestine of Lucilia sericata to LB medium, then shake the bacteria to the logarithmic phase at 37°C and 100 rpm; take out the medium and add it to a 10ml centrifuge tube, Centrifuge for 10 minutes, and collect the sediment as bacterial cells;
[0040] (2) According to a kind of novel Lucilia sericata-specific long-acting bacterial attractant as mentioned above, it is standby to take raw materials;
[0041] (3) Prepare bacterial growth matrix: mix soybean meal, fish meal, agar powder, potassium chloride, glycerin, urea and water evenly, then carry out autoclaving, then cool to 55°C to make bacterial growth matrix;
[0042] (4) Prepare Lucilia sericata attractant: add the bacterial cells cultivated over...
Embodiment 1
[0045]Embodiment 1 of the present invention discloses a novel Lucilia sericata-specific long-acting bacterial attractant, including: 5g Lucilia sericata intestinal bacterial thallus, 1g soybean powder, 1g fish meal, 1.0g agar powder, 0.5g chlorine dioxide in 100g Potassium chloride, 1g glycerin, 0.1g urea and the rest of water.
[0046] Embodiment 1 of the present invention also discloses a preparation method of a novel Lucilia sericata-specific long-acting bacterial attractant, specifically comprising the following steps:
[0047] (1) Preparation of bacterial cells: add Klebsiella oxytoca isolated from the intestine of Lucilia sericata to LB medium, then shake the bacteria to the logarithmic phase at 37°C and 100 rpm; take out the medium and add Put it into a 10ml centrifuge tube, centrifuge for 10 minutes, and collect the sediment as bacterial cells;
[0048] (2) According to a kind of novel Lucilia sericata-specific long-acting bacterial attractant as mentioned above, it i...
Embodiment 2
[0052] Embodiment 2 of the present invention discloses a novel Lucilia sericata-specific long-acting bacterial attractant, including components in 100g: 10g Lucilia sericata intestinal bacterial thallus, 5g soybean meal, 5g fish meal, 1.5g agar powder, 1g Potassium chloride, 3g glycerin, 0.5g urea and the rest of water.
[0053] Embodiment 2 of the present invention also discloses a preparation method of a novel Lucilia sericata-specific long-acting bacterial attractant, specifically comprising the following steps:
[0054] (1) Preparation of bacterial cells: add bacteria isolated from the intestine of Lucilia sericata to LB medium, then shake the bacteria to the logarithmic phase at 37°C and 100 rpm; take out the medium and add it to a 10ml centrifuge tube, Centrifuge for 10 minutes, and collect the sediment as bacterial cells;
[0055] (2) According to a kind of novel Lucilia sericata-specific long-acting bacterial attractant as mentioned above, it is standby to take raw ma...
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