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Method for extracting blood glucose-reducing polypeptide K from momordica charantia seeds

A technology of hypoglycemic polypeptide and extraction method, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., to achieve the effects of high product activity, simple extraction method and high extraction rate

Inactive Publication Date: 2020-07-28
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the extraction process of bitter gourd polypeptide K and the comparative study of the content of polypeptide K in different varieties of bitter gourd.

Method used

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  • Method for extracting blood glucose-reducing polypeptide K from momordica charantia seeds
  • Method for extracting blood glucose-reducing polypeptide K from momordica charantia seeds
  • Method for extracting blood glucose-reducing polypeptide K from momordica charantia seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for extracting hypoglycemic polypeptide K in bitter gourd seeds, comprising the steps of:

[0033] (1) Weigh 0.5g of bitter melon seed protein powder, put it into a test tube, then add 10mL of water, shake well, add NaOH solution (0.1M) to adjust the pH of the feed solution to 9.5, and the ratio of feed to liquid is 1:20, Place in an ultrasonic extraction device for 10 minutes of ultrasound (the power of the ultrasonic extraction device is 380W, and the frequency is 100kHz);

[0034] (2) Stand in a water bath at 50°C for 60 minutes, then centrifuge at 10,000 rpm for 10 minutes at 4°C;

[0035] (3) Take the supernatant, adjust the pH value to 3.0 with 1M HCl, let it stand for 30 minutes, centrifuge at 10,000 rpm for 10 minutes at 4°C, and collect the precipitate;

[0036] (4) Dissolve the obtained precipitate with 2 mL of NaOH solution (0.1 M, pH=9.0) to obtain a polypeptide K solution.

Embodiment 2

[0038] A method for extracting hypoglycemic polypeptide K in bitter gourd seeds, comprising the steps of:

[0039] (1) Weigh 0.5g of balsam pear seed protein powder, put it into a test tube, then add 5mL of water, shake well, add NaOH solution (0.1M) to adjust the pH value of the feed solution to 8.0, the ratio of feed to liquid is 1:10, and ultrasonically 10min (the power of the ultrasonic extraction device is 380W, and the frequency is 100kHz);

[0040] (2) Stand in a water bath at 40°C for 30 minutes, then centrifuge at 10,000 rpm for 10 minutes at 4°C;

[0041] (3) Take the supernatant, adjust the pH value to 3.0 with 1M HCl, let it stand for 30 minutes, centrifuge at 10,000 rpm for 10 minutes at 4°C, and collect the precipitate;

[0042] (4) Dissolve the obtained precipitate with 2 mL of NaOH solution (0.1 M, pH=9.0) to obtain a polypeptide K solution.

Embodiment 3

[0044] A method for extracting hypoglycemic polypeptide K in bitter gourd seeds, comprising the steps of:

[0045] (1) Weigh 0.5g of balsam pear seed protein powder, put it into a test tube, then add water, shake well, add NaOH solution (0.1M), the pH value of the seasoning solution is 10.0, the ratio of solid to liquid is 1:50, and ultrasonic for 10min ( The power of the ultrasonic extraction device is 380W, and the frequency is 100kHz);

[0046] (2) Stand in a water bath at 60°C for 120 minutes, then centrifuge at 10,000 rpm for 10 minutes at 4°C;

[0047] (3) Take the supernatant, adjust the pH value to 7.0 with 1M HCl, let it stand for 30 minutes, centrifuge at 10,000 rpm for 10 minutes at 4°C, and collect the precipitate;

[0048] (4) Dissolve the obtained precipitate with 2 mL of NaOH solution (0.1 M, pH=9.0) to obtain a polypeptide K solution.

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Abstract

The invention belongs to the technical field of extraction of natural active substances, and particularly relates to a method for extracting blood glucose-reducing polypeptide K from momordica charantia seeds. The method for extracting blood glucose-reducing polypeptide K from momordica charantia seeds comprises the following steps of (1) weighing momordica charantia seed protein powder, loading into a test tube, adding clear water, shaking up uniformly, regulating the pH value of the feed liquid with a NaOH solution, and carrying out ultrasonic treatment in an ultrasonic extraction device for10 minutes; (2) placing the test tube filled with the feed liquid in a water bath for extraction, and then placing the test tube in a centrifuge for centrifugation for 10 min; (3) taking the supernate, adjusting the pH value with 1M of HCL, standing for 30 minutes, centrifuging in the centrifuge for 10 minutes, and collecting the precipitate; and (4) dissolving the precipitate obtained in the step (3) with 2mL of NaOH solution to obtain the blood glucose-reducing polypeptide K solution. The extraction process of the blood glucose-reducing polypeptide K in the momordica charantia seeds is simple, the extraction rate of the blood glucose-reducing polypeptide K is high, the purity and activity of the obtained blood glucose-reducing polypeptide K product are relatively good, and a basis is provided for further screening out momordica charantia varieties with high polypeptide K content and developing momordica charantia blood glucose-reducing functional products.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural active substances, and in particular relates to an extraction method of hypoglycemic polypeptide K in bitter gourd seeds. Background technique [0002] Bitter melon (Momordica charantia L.) is an annual climbing herb of the genus Momordica cucurbitaceae. With high nutritional value and health care function, it is a kind of vegetable with the same source of medicine and food. In recent years, the planting area of ​​bitter gourd has gradually increased, and it has become the main vegetable variety and characteristic industry of "South Vegetable Transport North". Bitter melon is rich in peptides, polysaccharides, saponins, flavonoids and other biologically active ingredients, which have medicinal value such as lowering blood sugar, lowering blood fat, anti-tumor, anti-virus, enhancing immunity and preventing obesity. In recent years, with the improvement of people's living standards a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/30C07K1/14A61P3/10
CPCA61P3/10C07K14/415
Inventor 冯诚诚黄如葵黄玉辉陈小凤梁家作黄熊娟琚茜茜秦健苏健刘杏连
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI