Construction method for escherichia coli infected monolayer compact bovine mammary epithelial cell model

A technology of mammary gland epithelial cells and Escherichia coli, which is applied in the biological field and can solve problems such as endangering human and animal health and polluting the environment
CN111454879APending Publication Date: 2020-07-28CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-07-28

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Abstract

The invention provides a construction method for an escherichia coli infected monolayer compact bovine mammary epithelial cell model. The cell model is a monolayer compact bovine mammary epithelial cell model infected with escherichia coli is ATCC 25922, and during infection, the transepithelial electrical resistance value of the monolayer compact bovine mammary epithelial cell is 400 [omega]. cm<2>. The escherichia coli infected monolayer compact bovine mammary epithelial cell model provided by the invention can be used for detecting the adhesion rate of the escherichia coli infected monolayer compact bovine mammary epithelial cell and an influence of the escherichia coli infected monolayer compact bovine mammary epithelial cell for the expression of bovine mammary epithelial cell tight junction protein, and can be widely applied to related fields, including the screening of an injury mechanism of the escherichia coli induced mastitis for mammary tissues and mammary epithelial cells,and an antibiotic substitute.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for constructing a single-layer compact cow mammary gland epithelial cell model infected with Escherichia coli and its application. Background technique

[0002] Mastitis is a kind of mammary gland inflammation caused by bacterial infection. It is one of the common and extremely harmful diseases in the dairy farming industry. It is a persistent disease in the global dairy farming industry. And other characteristics, not only caused great suffering to animals, but also brought huge economic losses to dairy farms all over the world.

[0003] Escherichia coli is the main environmental pathogen that induces clinical mastitis in dairy cows, among which Escherichia coli ATCC25922 is an Escherichia coli strain isolated from mastitis cows, which can cause acute or hyperacute mastitis.

[0004] At present, the treatment of acute mastitis in cows mainly depends on the use of antibiot...

Claims

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