Polygenic lineage screening method of colletotrichum lindemuthianum

Abstract

The invention discloses preparation and application of a polygenic lineage screening database of colletotrichum. The invention provides application of substances represented by a) and b) in identification or distinguishing of colletotrichum species; the substance represented by a) is a substance for detecting the following gene sequences in a to-be-detected sample or a to-be-detected pathogenic bacteria: ITS, GAPDH, CHS-1, HIS3, ACT, TUB2, CAL, ApMat and GS; and the substance represented by b) is a substance for recording the existing colletotrichum species and ITS, GAPDH, CHS-1, HIS3, ACT, TUB2, CAL, ApMat and GS gene sequences in the various species. According to the invention, construction of an evolutionary tree and homology comparison are carried out by adoption of the ITS, GAPDH, CHS-1, HIS3, ACT, TUB2, CAL, ApMat and GS gene sequences; the colletotrichum lindemuthianum can be detected; at the quarantine port, a sample containing colletotrichum fungus can be screened rapidly andaccurately; spreading of bacteria along with importing, exporting, allocating and transporting of seeds and other propagation materials can be prevented; therefore, the health development of agricultural production in China is protected; and thus, the polygenic lineage screening method of colletotrichum lindemuthianum has important meanings.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to the preparation and application of a multigene pedigree screening database of the genus Neptonia, in particular to a multigene pedigree screening method for the bean anthracnose pathogen. Background technique [0002] At present, the genus Craniospora contains about 230 species with clear phylogenetic status, most of which are plant pathogenic fungi, which can attack a variety of crops, economic crops and ornamental plants, etc., forming a variety of diseases mainly anthracnose, causing huge economic loss. The genus Neptonia is one of the most frequently detected harmful phytopathogenic fungi at quarantine ports in my country. From 2007 to 2017, the detection rate of Neptonia accounted for about 7% of the total detection rate of phytopathogenic fungi, and the number of detections was the highest. Column number 4. It is not only a heavy task but also a big challenge for the por...

AI Technical Summary

Problems solved by technology

[0003] Currently, there are some molecular methods for the specific detection and identification of some species of Neptonia spp., however, these methods can only detect one pathogenic fungus at a time

So far, none have been used for screening of fungi at the genus level of Trichoderma

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