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Method for detecting abundance change and genotype distribution of intestinal tract Akk of patients with Parkinson's disease and other diseases

A technology for Parkinson's disease and genotyping, applied in the direction of microbial-based methods, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problem of being able to distinguish few strains at the strain level

Pending Publication Date: 2020-07-31
GUANGZHOU KANGZE MEDICAL TECH CO LTD +1
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Problems solved by technology

Another limitation is that currently used 16S rRNA-based sequencing enables taxonomic identification at most to the genus level and rarely differentiates to the strain level.

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  • Method for detecting abundance change and genotype distribution of intestinal tract Akk of patients with Parkinson's disease and other diseases
  • Method for detecting abundance change and genotype distribution of intestinal tract Akk of patients with Parkinson's disease and other diseases
  • Method for detecting abundance change and genotype distribution of intestinal tract Akk of patients with Parkinson's disease and other diseases

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Embodiment Construction

[0043] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, and the schematic embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0044] In the previous research work of the inventor, 37 human intestinal Akk strains have been isolated and divided into three genotypes I-III. Through PCA and CAZy spectrum analysis, it was found that the genomes of AkkⅠ, AkkⅡ, and AkkⅢ are quite different.

[0045] AkkII and AkkIII metabolize carbohydrates more diversely than AkkI. Animal experiments in previous studies have preliminarily verified that Akk bacteria can alleviate learning and cognitive impairment, and it has also been found that Akk I subtype (GP01 strain) has a stronger ability to decompose medium components to produce acetic acid, propionate and isovaleric acid than the model strain of the same Akk I subtype...

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Abstract

The invention discloses a method for detecting abundance change and genotype distribution of intestinal tracts Akk of patients with Parkinson's disease (PD) and other diseases. The method comprises the following steps: collecting an excrement sample; extracting excrement DNA; and detecting relative abundance of AkkI, AkkII and AkkIII in the excrement DNA through real-time fluorescence quantitativePCR (RT-qPCR). The method has the advantages that: a method for quickly analyzing the distribution of three Akk subtypes in the intestinal tract is established by utilizing the RT-qPCR, the correlation between the abundance change of the Akk subtypes and the PD and other diseases can be preliminarily discussed, a foundation is laid for subsequent discussion of action mechanisms of different Akk subtypes, and a new thought can be possibly provided for prevention and treatment of the PD and other diseases in the future.

Description

technical field [0001] The invention relates to the technical field of intestinal bacteria detection, in particular to the detection technology of intestinal bacterial abundance and genotype distribution. Background technique [0002] Parkinson's disease (PD) is a degenerative disease characterized by abnormal deposition of α-synuclein in the brain and outer nervous system. Studies have shown that the imbalance of intestinal flora will lead to the deposition of α-synuclein in the enteric nervous system, and migrate along the brain-gut axis to the brain and deposit, and finally lead to the occurrence of PD. Therefore, some scholars speculate that the early onset of PD may be in the intestinal tract, which is caused by the imbalance of flora. Therefore, exploring the interaction relationship between the brain-gut-microbial axis and clarifying the causal relationship and mechanism between the change of gut flora and the pathogenesis of PD will help to deeply understand the pat...

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689C12Q1/686C12Q2561/113C12Q2563/107C12Q2545/114C12Q2521/107
Inventor 彭永正黄东泉易江丰
Owner GUANGZHOU KANGZE MEDICAL TECH CO LTD
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