Periphyton sampling method suitable for river with high flow rate and high sand content

Abstract

The invention relates to a periphyton sampling method suitable for a river with a high flow rate and a high sand content. A sampler composed of a base plate and a cover plate which are hinged to eachother is adopted, the base plate and the cover plate can be locked through a lock catch so that the base plate and the cover plate can be attached together, a hollowed-out sampling window is formed inthe cover plate, and a handle is arranged at the hinged position of the base plate and the cover plate. The periphyton sampling method comprises the following steps of placing a polyester film at a sampling window of the sampler, connecting a handle by using a tether, lowering the sampler at a preset place of a river, adjusting the sampler to a predetermined water layer, fixing the tether, and placing for a specified time, lifting the sampler out of the water surface, cutting the polyester film with a preset area in the sampling window, and collecting attached periphytons into the sample bottle. The method can be applied to collecting the periphyton in river sections with a high silt content and a high flow and a high flow rate.

Description

technical field [0001] The invention relates to a method for sampling periphytons suitable for rivers with high velocity and high sediment content, and belongs to the technical field of periphyton monitoring. Background technique [0002] Epiphytes, namely periphytes, refer to groups of organisms that grow on the surface of various substrates submerged in water. In recent years, the study of periphytes has been paid more and more attention because of their large primary production capacity, and they can be used in environmental protection to indicate the pollution level of water bodies (such as rivers, lakes, reservoirs, and oxidation ponds, etc.) . [0003] At present, sampling methods for epibiotic monitoring are mainly divided into two categories: natural matrix sampling and artificial matrix sampling. Among them, the sampling of natural substrates is carried out by using animals, plants, rocks and wood blocks in the water. Although this method is convenient for samplin...

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Problems solved by technology

However, the polyester film will float freely with the water and be easily entangled during sampling. If a large area is sampled, it will be easily lost by the water flow, and reducing the sampling area will not be able to meet the standard sampling volume (SL167-2014 "Reservoir Fishery Resources Investigation Specification" Requirements for counting individual requirements of more than 100)

At the same time, the traditional polyester film method is easy to cause the film strips to fall off and be damaged during the process of recycling polyester film, and it is necessary to repeatedly fix the film strips one by one for replacement.

[0006] The above two sampling methods have high requirements for fixation, and it is necessary to avoid torrents and eddies in rivers, and in rivers with high flow velocity and high sediment content, affected by sediment, the floating device installed with glass slides is very easy to deposit mud Blockage of sand or sundries will block sampling, etc., making the representativeness of collected samples poor, while polyester film will be more easily damaged and lost under the action of sand and water erosion, making sample collection difficult

That is to say, the existing sampling methods for periphyton cannot be applied in rivers with high flow velocity and high sediment concentration, and the requirements for sampling locations are relatively high, and torrents and eddies must be avoided in rivers

[0007] In addition, the traditional sampling method is limited by slides (the counting area of ​​slides is usually 4cm) 2 ) and polyester film strips, for rivers with low algae density, such as the lower reaches of the Yangtze River, in order to ensure the sampling volume (as mentioned above, according to the standard sampling volume requirement of more than 100), it is necessary to accumulate multiple calculations, microscopic inspection huge workload

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