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Metaproteome mass spectrometric data analysis method based on MetaPro-IQ method

A proteome and mass spectrometry data technology, applied in the field of bioinformatics, can solve problems such as high software and hardware requirements, long running time, redundant false positive results, etc., to achieve reduced volume, low hardware configuration requirements, and low processing time short-term effect

Pending Publication Date: 2020-08-04
上海中科新生命生物科技有限公司
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Problems solved by technology

However, the full species database used by the latter is generally large in size. For example, the NR full database is as high as hundreds of GB, which requires high software and hardware, takes a long time to run, and has high redundancy and false positives in the results.

Method used

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  • Metaproteome mass spectrometric data analysis method based on MetaPro-IQ method
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  • Metaproteome mass spectrometric data analysis method based on MetaPro-IQ method

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Embodiment Construction

[0038] The technical solutions of the present invention will be described below in conjunction with the accompanying drawings and embodiments.

[0039] A method for analyzing metaproteome mass spectrometry data based on the MetaPro-IQ method, comprising the steps of:

[0040] Step S1 preliminarily streamlines the database: use X! for each piece of data to be tested separately. Tandem Alanine software searches and matches in the original database respectively, and the software parameters are set as follows:

[0041] parameter value fragment monoisotopic mass error 20 parent monoisotopic mass error plus 5 parent monoisotopic mass error minus 5 modification mass 57.022@C potential modification mass 16.0@M maximum missed cleavage sites 2 maximum valid expectation value 0

[0042] No result screening is performed during the search and matching process. After searching and matching, all matching results of each piece of data ...

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Abstract

The invention aims to provide a metaproteome mass spectrometric data analysis method based on a MetaPro-IQ method. Based on MetaPro-IQ, a set of method for quickly filtering low-matching-degree protein for multiple times and reducing the size of a database is established, so that the simplified database can be normally used in conventional database searching software. The method is simple and convenient to operate, and has the advantages that a protein database with a super-large volume can be processed, the processing process is short in time consumption, advanced hardware configuration is not needed, and more proteins can be identified.

Description

technical field [0001] The invention relates to the technical field of biological information, in particular to a metaproteome mass spectrometry data analysis method based on the MetaPro-IQ method. Background technique [0002] With the rapid development of mass spectrometers and bioinformatics, scientific research at the protein level has entered the stage of high-throughput and big data, and research methods have emerged in an endless stream. Among them, the metagenome and metaproteome represented by intestinal microorganisms and environmental microorganisms are very difficult to identify by mass spectrometry due to the highly complex composition and distribution of sample species. [0003] The routine processing procedures for studying metaproteomic mass spectrometry data generally need to rely on high-quality metagenomic data results, or blindly search public databases such as NCBI's NR full library directly. The former is to splice and annotate the amino acid sequence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B50/00G16B30/10G01N27/62
CPCG16B50/00G16B30/10G01N27/62
Inventor 秦聪
Owner 上海中科新生命生物科技有限公司
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