An injectable self-healing hydrogel with adjustable mechanical properties and its preparation method and application
A self-healing, hydrogel technology, applied in medical science, pharmaceutical formulations, bandages, etc., can solve the problems of no further research on hemostasis and promotion of wound healing, prolonged gel time, and inability to inject smoothly, so as to promote wound healing. , Expand the scope of application, the effect of adjustable mechanical properties
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Embodiment 1
[0054] Preparation of oxidized hyaluronic acid (OHA):
[0055] To 200 mL of 10 mg / mL hyaluronic acid aqueous solution, add 5 mL of 0.5 mol / L sodium periodate aqueous solution dropwise, and stir for 2 h in the dark; then add 1 mL of ethylene glycol, and continue stirring for 1 h; After water dialysis for 3 days, it was freeze-dried, and the molecular weight cut-off of the dialysis bag was 8-14KDa to obtain oxidized hyaluronic acid (OHA).
[0056] The hydrogen nuclear magnetic spectrum of the above-mentioned oxidized hyaluronic acid is as follows: figure 1 As shown, compared with hyaluronic acid (HA), a new aldehyde group characteristic peak appears in the OHA NMR spectrum at 4.9-5.0ppm, indicating that OHA was successfully synthesized.
Embodiment 2
[0058] Preparation of succinyl chitosan (NSC):
[0059] Chitosan is dispersed in dimethyl sulfoxide containing succinic anhydride, so that the concentration of chitosan in the reaction system is 50mg / mL; the amino group of chitosan: the molar ratio of succinic anhydride is 1:(1-2 ), stirred and reacted at 60°C for 1.5-4h and then centrifuged; dispersed the precipitate in water, adjusted the pH value to 10-12 with sodium hydroxide solution to form a yellow solution; added acetone to produce a precipitate; centrifuged to collect the precipitate, washed with acetone, and dried Succinyl chitosan (NSC) was obtained.
[0060] The proton nuclear magnetic spectrum of above-mentioned succinyl chitosan is as figure 2 As shown, compared with chitosan (CS), the characteristic peak of new methylene hydrogen appears in the NSC NMR spectrum at 2.40-2.49ppm, indicating that NSC was successfully synthesized.
Embodiment 3
[0062] A method for preparing an injectable self-healing hydrogel with adjustable mechanical properties, comprising the following steps:
[0063] (1) The OHA prepared in Example 1 was dissolved in the phosphate buffer solution of pH=7.4 to obtain a 60 mg / mL OHA solution; calcium chloride (CaCl 2 ) was dissolved in water to obtain 120 mg / mL calcium chloride aqueous solution; OHA solution and calcium chloride aqueous solution were mixed, wherein the mass ratio of OHA and calcium chloride was 1:0.1 to obtain OHA composite solution 1;
[0064] (2) Dissolve the NSC prepared in Example 2 in a phosphate buffer solution with pH=7.4 to obtain a 60 mg / mL NSC solution;
[0065] (3) Mix the OHA composite solution 1 in step (1) with the NSC solution in step (2), the mass ratio of the two is 1:1, and the cross-linking reaction is 30-60 seconds at 25-37°C to obtain NSC- OHA-Ca2 hydrogel.
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