Multi-photon fluorescence microscopic imaging system with ultra-fast time resolution and low excitation threshold

Abstract

The invention discloses a multi-photon fluorescence microscopic imaging system with ultra-fast time resolution and a low excitation threshold. The multi-photon fluorescence microscopic imaging systemcomprises a femtosecond laser, an adaptive femtosecond pulse shaping module, a fluorescence microscopic imaging module, a compressed sensing ultra-fast imaging module and a computing communication module. According to the invention, a target femtosecond pulse after time domain shaping is obtained through the femtosecond laser and the adaptive femtosecond pulse shaping module; a fluorescence microscopic signal is obtained by the fluorescence microscopic imaging module, sparse coding two-dimensional digital image data are generated by the compressed sensing ultra-fast imaging module, and targetsignal reconstruction is carried out on the sparse coding two-dimensional digital image data by the computing communication module. According to the invention, the femtosecond laser pulse is subjectedto waveform shaping, phase modulation and sparse coding of fluorescence information, so that the excitation threshold of a multi-photon fluorescence process is reduced; and the fluorescence lifetimeis estimated by using a sparse coding model, so that single-pulse fluorescence microscopic imaging with a low multi-photon excitation threshold characteristic and ultra-fast time resolution of a single pulse is realized.

Description

technical field [0001] The invention relates to the technical fields of laser pulse shaping and computational optical imaging, in particular to a multiphoton fluorescence microscopic imaging system with ultrafast time resolution and low excitation threshold. Background technique [0002] Physically, the multiphoton fluorescence microscope is essentially different from the general single-photon fluorescence process. Multiphoton fluorescence excitation means that a fluorescent molecule absorbs two or more long-wavelength photons at the same time, and excites a fluorescent molecule that satisfies energy conservation and momentum. Conserved short-wavelength photons. Therefore, compared with ordinary single-photon fluorescence technology, multi-photon fluorescence technology has many outstanding advantages for biological fluorescence microscopy imaging, such as deep penetration depth, high resolution, and high signal-to-noise ratio. However, since the excitation process of N pho...

AI Technical Summary

Problems solved by technology

Due to the huge amount of information in the video imaging process, the physical limit of information transmission of electronic devices greatly limits the repetition rate of the video continuous sampling process, which poses a great challenge to the continuous shooting of the scanning fluorescence lifetime microscope FLIM.

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