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Process for separating mixture with measurement of purity or yield on intermediate tank

A technology for mixtures and purity, applied in the field of chromatography for separating mixtures, which can solve problems such as hindering measurement and complex analysis equipment.

Active Publication Date: 2020-08-14
ノヴァセププロセスソリューションズ
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to complicating the analytical setup, these purging, cleaning, and preparation steps can prevent measurements being made during each cycle

Method used

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  • Process for separating mixture with measurement of purity or yield on intermediate tank
  • Process for separating mixture with measurement of purity or yield on intermediate tank
  • Process for separating mixture with measurement of purity or yield on intermediate tank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0398] The following examples illustrate the invention without limiting it.

[0399] In subsequent embodiments 1 to 5, the chromatographic simulation model is used, and its parameters are as follows:

[0400] - Substances present in the mixture to be separated: dextran, glucose and fructose with a degree of polymerization (order) greater than or equal to 2;

[0401] - stationary phase: resin XA2004 / 30Ca from Novasep;

[0402] - mobile phase: water;

[0403] - Elution temperature: between 55°C and 65°C.

[0404] These concentration plots representing loading pulses, high concentration saturation, and low concentration saturation at a flow rate of 20 mL / min in a column 2 m long and 8.5 cm in diameter are shown in Figure 5.A , 5.B and 5.C. These data make it possible to use, for example, in the handbook "Preparative Chromatography of Fine Chemicals and Pharmaceutical Agents (Preparative Chromatography of Fine Chemicals and Pharmaceutical Agents)", Henner Schmidt-Traub, Wile...

Embodiment 3

[0406] The method used in Example 3 was SMB with a total column height of 8 meters.

[0407] In the following examples 6 to 7, real chromatographic separations were carried out on a pilot plant using the following parameters:

[0408] - Substances present in the mixture to be separated: dextran, glucose and fructose with a degree of polymerization greater than or equal to 2;

[0409] - stationary phase: resin XA2004 / 30Ca from Novasep;

[0410] - mobile phase: water;

[0411] - Elution temperature: between 55°C and 65°C;

[0412] - The pilot plant consists of 4 chromatographic columns with a length of 2 meters and a diameter of 8.5 centimeters;

[0413] - The flow rate of product and eluent is between 20 and 30 mL / min;

[0414] - The method used is SSMB with a total column height of 8 meters.

Embodiment 1

[0416] A simulation of the chromatographic method was performed as described above.

[0417] Stabilize the system between cycles 1 and 10, fixing and keeping the volumes of the different regions constant. The volume of mobile phase injected was 0.18 BV (bed volume).

[0418] Starting from the 10th cycle, use +0.17 (deviation of the target position from the characteristic point of the adsorption volume divided by the cycle volume, relative to the average eluent injection position) located in the region 4 and -0.17 in the region 1 (the target position and The deviation of the characteristic point of the desorption volume divided by the cycle volume, relative to the target position of the characteristic point of the average eluent injection position) to adjust the area. This corresponds to the target position of the low concentration feature point at the mean eluent injection point.

[0419] Then, without limiting the volume of the mobile phase, the method using two characteris...

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Abstract

The invention relates to a process for separating a mixture in a device. The device comprises a first system comprising a plurality of chromatography columns, an outlet line for collecting a raffinateand an outlet line for collecting an extract; at least one second system placed downstream of the first system; and at least one tank fed by one of the outlet lines from the first system, and feedingthe second system with a continuous flow, and the process successively comprises, in a cyclical manner, in a given part of the first system, a step of collecting a raffinate, a step of injecting themixture to be separated, a step of collecting an extract and a step of injecting mobile phase, wherein the process comprises, moreover, measuring the purity and / or the yield of at least one collectedfraction chosen from the extract and the raffinate, and the purity and / or yield measurement comprises the following steps of determining the concentration of at least two species of the mixture to beseparated in the fraction collected in the tank; determining the purity and / or the yield of at least one species of the fraction collected in the tank.

Description

technical field [0001] The invention relates to a chromatographic method for separating mixtures. Background technique [0002] Chromatography is a separation technique based on differences in the distribution of compounds in a mixture between a mobile phase and a stationary phase. Separation of compounds by diafiltration of a mobile phase containing liquid, gaseous, or supercritical solvents in a device (called a column or cell) filled with a stationary phase, where all or some of the compounds have a different diafiltration compared to the rest When the speed is high, the separation is achieved. The method is used as an analytical technique to identify and quantify compounds in a mixture. It can also be used as a purification technique. [0003] Purification of molecules is carried out using various chromatographic methods as required. Some chromatographic methods are described in the literature LargeScale Adsorption and Chromatography, P.C. Wankat, CRC press, Boca-Rat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/18C13K1/00C13K11/00
CPCB01D15/1828C13K1/00C13K11/00
Inventor 塞德里克·普里厄埃里克·瓦莱里
Owner ノヴァセププロセスソリューションズ