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Genetic molecular marker related to gastric cancer auxiliary diagnosis and application thereof

A technology of molecular markers and auxiliary diagnosis, applied in the field of genetic molecular markers, can solve the problem of low prediction efficiency

Pending Publication Date: 2020-08-18
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Through the above analysis, the existing problems and defects of the existing technology are: most of the genetic models of gastric cancer in the past were constructed using GWAS loci, and their prediction efficiency was low, and the effect of the genetic prediction model could not be maximized

Method used

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  • Genetic molecular marker related to gastric cancer auxiliary diagnosis and application thereof
  • Genetic molecular marker related to gastric cancer auxiliary diagnosis and application thereof
  • Genetic molecular marker related to gastric cancer auxiliary diagnosis and application thereof

Examples

Experimental program
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preparation example Construction

[0046] (4) Preparation of an auxiliary diagnostic kit for gastric cancer: an auxiliary diagnostic kit was constructed based on the SNP genetic markers screened above.

[0047] The technical effects of the present invention will be described in detail below in conjunction with experiments.

[0048] experimental method:

[0049] 1. Collect research objects

[0050] (1) Cases of gastric cancer confirmed by pathology;

[0051] (2) Healthy control;

[0052] The present invention included 1835 gastric cancer cases and 2613 healthy controls (training set: 1625 cases and 2100 controls; test set: 210 cases and 513 controls) to identify risk sites.

[0053] 2. The phenol-chloroform method was used to extract the genomic DNA from the peripheral blood, which was operated according to the conventional method. Usually 20-50ng / μl DNA can be obtained, and the purity (ratio of UV 260OD to 280OD) is 1.6-2.0.

[0054] 3. Genome-wide SNPs detection in the training set and preliminary screeni...

Embodiment 1

[0085] The collection of embodiment 1 sample and the arrangement of data

[0086] Training set: The inventor obtained the GWAS data of gastric cancer in the Chinese population from the Genotype and Phenotype Database (dbGaP, phs000361.v1.p1). Including 1625 cases and 2100 healthy controls.

[0087] Test set: The inventor collected gastric cancer blood samples from local hospitals in Nanjing, Shanghai, and Xuzhou from 2010 to 2018, and sorted them out, including 210 gastric cancer samples and 513 control cases who underwent physical examination in the hospital at the same time. epidemiological and clinical data.

Embodiment 2

[0088] Example 2 Whole Genome Scanning of SNPs in Peripheral Blood DNA in Training Set

[0089] In eligible 1625 gastric cancer cases and 2100 healthy controls, Illumina Human 660W-Quad Chips were used for genotyping. Specific steps are as follows:

[0090] (1) Configuration of the lysate: 40 portions were prepared, that is, 219.72 g of sucrose, 2.02 g of magnesium chloride, and 20 ml of Triton X-100 (amresco 0694) were mixed, and the volume was adjusted to 2000 ml with TrisHcl solution. (2) Add the lysate to the peripheral blood in the 2ml cryopreservation tube, and mix evenly by inverting. (3) Removal of erythrocytes: Fill the 5ml centrifuge tube to the 4ml mark with the lysate, mix well, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of lysate to the precipitate, mix again, wash once, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. (4) Preparation of extract: each 300ml contains 122.5ml 0.2M sodium chloride, 14.4ml 0.5M ethy...

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Abstract

The invention belongs to the technical field of gene engineering, and discloses a genetic molecular marker related to gastric cancer auxiliary diagnosis and an application thereof and totally 42 genetic loci are provided. According to the invention, a genetic risk prediction model is constructed. The invention provides an application of specific primers of the SNP marker and an application of thespecific primers in preparation of a gastric cancer auxiliary diagnosis kit, and the gastric cancer auxiliary diagnosis kit. The genetic marker kit for assisting in gastric cancer diagnosis is expected to lay a foundation for screening and individualized prevention and treatment of high-risk groups of gastric cancer in China, and a decision basis is provided. The SNP genetic marker is different from a traditional biomarker, and is good in stability and easy to detect. The gastric cancer auxiliary diagnosis kit is helpful for clinicians to quickly grasp the genetic risk of patients suffering from gastric cancer, perform early diagnosis and individualized prevention and treatment, and provide a decision basis for screening and intervention of high-risk groups of gastric cancer.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a genetic molecular marker related to the auxiliary diagnosis of gastric cancer and its application. Background technique [0002] At present, gastric cancer is a common malignant tumor, its incidence rate accounts for the sixth of all tumors in the world, and it has obvious regional characteristics, especially in East Asia such as China, Japan, and South Korea. According to statistics, in 2015, there were about 4.29 million new cancer cases in China, and 2.81 million cancer patients died of cancer. Among them, gastric cancer had 680,000 cases and 500,000 deaths, ranking second among all tumors and seriously endangering human health. In recent years, with my country's economic development and social progress, the incidence and mortality of gastric cancer have been declining year by year. However, due to the difficulty in early detection of gastric cancer, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113C12N15/11
CPCC12Q1/6886C12Q2600/156Y02A50/30
Inventor 张正东王美林辛俊逸杜牧龙邬燕玲黎书炜储海燕
Owner NANJING MEDICAL UNIV
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