Composition containing porphyridium polypeptide and preparation method of composition

A technology of Porphyridium porphyridum polypeptide and Porphyridium porphyridum, which is applied to the preparation method of peptides, medical preparations containing active ingredients, drug combinations, etc., can solve the problems such as the undeveloped Porphyrococcus medicinal use, and achieve the promotion of wound healing, Reduced wound opening time and high preparation efficiency

Active Publication Date: 2020-08-25
MARINE MEDICAL RES INST OF GUANGDONG ZHANJIANG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At this stage, the research on Porphyridium is mostly focused on its immunity-enhancing, anti

Method used

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  • Composition containing porphyridium polypeptide and preparation method of composition
  • Composition containing porphyridium polypeptide and preparation method of composition
  • Composition containing porphyridium polypeptide and preparation method of composition

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preparation example Construction

[0031] The preparation method of the composition containing the above-mentioned Porphyridium algae polypeptide is:

[0032] The steps for obtaining the Porphyridum polypeptide: take the Porphyridum thawing repeatedly, then ultrasonically break it, and centrifuge the obtained algae liquid to get the supernatant; conduct vertical electrophoresis on Tricine gel to separate the polypeptide below 5KDa, which is Porphyridium polypeptide;

[0033] Or, take Porphyridum algae after homogenization, sterilization, filtration, centrifugation, collect the supernatant, and use ultrafiltration membrane system with 0.001-0.1μm microporous membrane, the molecular weight cut-off is 1KDa, and the operating pressure difference is 0.1-0.5 MPa, obtain the Porphyridium polypeptide;

[0034] Mixing step: mixing the Porphylococcus polypeptide with the substrate; obtaining a composition containing the Porphylococcus polypeptide.

Embodiment 1

[0036] Preparation of Porphyridium algae polypeptide: take Porphyridum algae, add sterile distilled water, freeze-thaw repeatedly (-20°C overnight and room temperature for 3 times), then ultrasonically break for 30 minutes, stop for 5 minutes, repeat 5 times; the obtained algae liquid is centrifuged and taken Supernatant; Tris hydroxymethylglycine (Tricine) gel vertical electrophoresis for separation, get the polypeptide band below 5KDa, it is Porphylococcus polypeptide; cut off the target band and submit it for inspection, and analyze it by liquid chromatography-mass spectrometry. There are two polypeptides: 6 peptides (720.87Da), the sequence IFNLSK is shown in SEQ ID NO.1, and 17 peptides (1862.18Da), the sequence FTRPREVAAAALPPPSPR is shown in SEQ ID NO.2.

Embodiment 2

[0038] The preparation of Porphyridum polypeptide: Take Porphyridum, add 1-6 times the volume of sterile water, homogenize, sterilize, filter, centrifuge, collect the supernatant, and use 0.001-0.1μm microporous membrane for ultrafiltration Membrane system ultrafiltration, the molecular weight cut-off is 1KDa, the operating pressure difference is 0.1-0.5MPa, liquid chromatography-mass spectrometry analysis, it is known that it is two polypeptides: 6 peptides (720.87Da), the sequence IFNLSK such as SEQ ID NO.1 As shown, 17 peptides (18 62.18Da), the sequence FTRPREVAAAALPPPSPR is shown in SEQ ID NO.2.

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Abstract

The invention discloses a composition containing porphyridium polypeptide and a preparation method of the composition. The composition comprises the following effective components in parts by weight:0.1-0.9 part of porphyridium polypeptide and 99.1 to 99.9 parts of a substrate. The preparation method comprises the following steps: an obtaining step of the porphyridium polypeptide: repeatedly freezing and thawing porphyridium, performing ultrasonic crushing, centrifuging an obtained porphyridium liquid, taking supernate, performing separating by vertical electrophoresis to obtain polypeptide with molecular weight of 5Kda or below, namely the porphyridium polypeptide; or taking the porphyridium, performing homogenizing, sterilizing, filtering and centrifuging, collecting supernatant, and performing ultrafiltration by using an ultrafiltration membrane system with a microporous membrane of 0.001-0.1 [mu]m to obtain the porphyridium polypeptide with the molecular weight cutoff 1KDa; and amixing step: mixing the porphyridium polypeptide with the matrix in parts by weight to obtain the composition containing the porphyridium polypeptide. The composition can promote wound healing, reducewound opening time and facilitate rehabilitation after body surface damage.

Description

technical field [0001] The invention relates to a composition containing a porphyridum polypeptide and a preparation method thereof, belonging to the technical field of biomedical preparations. Background technique [0002] Porphyridium, as a relatively primitive single-celled algae of the Rhodophyta, can produce many biologically active substances, such as phycobiliproteins, polyunsaturated fatty acids and exopolysaccharides, and is currently mainly used as bait for juvenile shrimp. However, current studies believe that Porphyridium algae has high medicinal value. In recent years, it has been newly discovered that Porphyridium algae preparations have special effects in the prevention and treatment of cardiovascular and cerebrovascular diseases and cancer. At present, the research on Porphyridium is mostly focused on improving immunity, anti-cancer and anti-thrombotic activities, but the medicinal development of Porphyridum has not yet been carried out. With the developmen...

Claims

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Application Information

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IPC IPC(8): A61K38/08A61K38/10A61K36/04A61K9/06A61P17/02C07K7/06C07K7/08C07K1/14C07K1/26C07K1/34
CPCA61K38/08A61K38/10A61K36/04A61K9/06A61K9/0014A61K47/36A61P17/02C07K7/06C07K7/08A61K2300/00
Inventor 张海涛余华军伍俊罗辉谢文熙
Owner MARINE MEDICAL RES INST OF GUANGDONG ZHANJIANG
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