Method for producing prodigiosin based on PNTs

A technology for producing prodigiosin and prodigiosin, which is applied in the biological field and can solve the problems of low yield and hindering the industrialization process of microbial fermentation
CN111575221AActive Publication Date: 2020-08-25JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2020-08-25

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Abstract

The present invention discloses a method for producing prodigiosin based on PNTs, belongs to the technical field of biology, and provides serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT capable of high production of prodigiosin. The serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT are obtained by respectively connecting nucleotide fragments shownin SEQ ID No.11 at 3' ends of prodigiosin synthesis gene clusters pigF and pigN of the serratia marcescens respectively. The serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT are respectively inoculated into a fermentation culture medium for fermentation for 96 h, so that the production of the prodigiosin in fermentation liquid is as high as 7.55 g / L and 8.37 g / L, respectively.
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Description

Technical field

[0001] The invention relates to a method for producing Prodigiosin based on PNTs, and belongs to the field of biotechnology. Background technique

[0002] Prodigosin is a kind of natural red pigment containing tripyrrole skeleton structure, which is mainly produced by some microorganisms. It has anti-cancer, immunosuppressive, anti-insect and other biological activities. It can be used as immunosuppressant and anti-cancer drugs to develop. Wide attention of researchers.

[0003] At present, the main methods of producing Prodigiosin are chemical synthesis and microbial fermentation. Among them, the chemical synthesis method is mainly to obtain prodigiosin through the method of series conjugate addition and high temperature dehydrogenation. However, due to the complexity and difficulty of the pathway and low yield, it is difficult for chemical synthesis to achieve large-scale industrial production. The principle of microbial fermentation is mainly to obtain prodigi...

Claims

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