Method for producing prodigiosin based on PNTs

Abstract

The present invention discloses a method for producing prodigiosin based on PNTs, belongs to the technical field of biology, and provides serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT capable of high production of prodigiosin. The serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT are obtained by respectively connecting nucleotide fragments shownin SEQ ID No.11 at 3' ends of prodigiosin synthesis gene clusters pigF and pigN of the serratia marcescens respectively. The serratia marcescens engineered bacteria JNB5-1 / pigFPNT and JNB5-1 / pigNPNT are respectively inoculated into a fermentation culture medium for fermentation for 96 h, so that the production of the prodigiosin in fermentation liquid is as high as 7.55 g / L and 8.37 g / L, respectively.

Description

Technical field [0001] The invention relates to a method for producing Prodigiosin based on PNTs, and belongs to the field of biotechnology. Background technique [0002] Prodigosin is a kind of natural red pigment containing tripyrrole skeleton structure, which is mainly produced by some microorganisms. It has anti-cancer, immunosuppressive, anti-insect and other biological activities. It can be used as immunosuppressant and anti-cancer drugs to develop. Wide attention of researchers. [0003] At present, the main methods of producing Prodigiosin are chemical synthesis and microbial fermentation. Among them, the chemical synthesis method is mainly to obtain prodigiosin through the method of series conjugate addition and high temperature dehydrogenation. However, due to the complexity and difficulty of the pathway and low yield, it is difficult for chemical synthesis to achieve large-scale industrial production. The principle of microbial fermentation is mainly to obtain prodigi...

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Problems solved by technology

[0004] However, there are still certain defects in the existing microbial fermentation method, among which, the low yield is the most important defect hindering the industrialization process of the microbial fermentation method

For example, Kim et al. produced prodigiosin by inoculating Hahella chejuensis KCTC 2396 into the 2216 medium of marine bacteria broth for fermentation. However, using this method to ferment for 40 hours, only the concentration of prodigiosin in the fermentation broth The yield reaches 28mg / L (specific references: Kim S J, Lee H K, Lee Y K, et al. Mutant selection ofHahella chejuensisKCTC 2396 and statistical optimization of medium components for prodigiosin yield-up[J]. Journal of Microbiology, 2008, 46(2): 183-188.); Martha Ingrid Gutiérrez-Román et al. inoculated Serratia marcescens CFFSUR-B4 into peanut-based medium for fermentation to produce prodigiosin, but using this method to ferment for 24 hours, only the prodigiosin in the fermentation broth could be made The production of bacteriocin reached 40.6 μg / mL (see references for details: Martha Ingrid Gutiérrez-Román, Francisco Holguín-Meléndez, Bello-Mendoza R, et al. Production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens[J ].World Journal of Microbiology and Biotechnology,2012,28(1):p.145-153.)

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