Application of aesculetin in prevention and treatment of plant oomycete diseases

A technology of acetin and phytopathogens, which is applied in the fields of application, plant growth regulator, botany equipment and methods, etc., can solve the problems such as the absence of acetaminophen inhibitory effect, achieve the problem of alleviating drug resistance, less environmental pollution, low residual effect

Inactive Publication Date: 2020-08-28
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report on the inhibitory ef...

Method used

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  • Application of aesculetin in prevention and treatment of plant oomycete diseases
  • Application of aesculetin in prevention and treatment of plant oomycete diseases
  • Application of aesculetin in prevention and treatment of plant oomycete diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Example 1: Inhibition of Phytophthora capsici, Phytophthora sojae, Phytophthora infestans, Phytophthora nicotiana, Pythium melon, Pythium ultima and Peronospora grapes mycelial growth of agaricin B.

[0015] Test method: The mycelium growth rate method was used to test the indoor toxicity of 7 kinds of oomycetes including Phytophthora capsici, Phytophthora soybean, Phytophthora infestans, Phytophthora tobacco, Pythium melon and fruit, Pythium ultima and Peronospora grapes . Using methanol as a solvent, prepare the test teretin B as a 20 mg / mL mother solution, add V8 medium and mix well, and use the doubling dilution method to make a final concentration of 0.3125, 0.625, 1.25, 2.5, 5 and 10 μg / mL The culture medium plate of teretin B, and methanol solvent was added as the control. Use a sterilized hole puncher with a diameter of 5 mm to punch bacteria cakes from the edge of the colonies of 7 kinds of oomycetes including Phytophthora capsici and insert them into the cult...

Embodiment 2

[0019] Example 2: Inhibition of spore germination of Phytophthora capsici, Phytophthora soybean, Phytophthora infestans, Phytophthora nicotiana, Pythium melon, Pythium ultima and Peronospora viticolae of agaricin B.

[0020] Test method: use a 5 mm diameter sterilized hole puncher to activate oomycete colonies of 7 kinds of oomycetes including Phytophthora capsici, Phytophthora soybean, Phytophthora infestans, Phytophthora tobacco, Pythium melon and fruit, Pythium ultima and Peronospora grape Take the bacteria cake from the edge and insert it into the V8 liquid medium plate. Place the plate in a constant temperature incubator at 25°C for dark culture for 2-4 days, pour out the V8 liquid medium gently (be careful not to pour out the hyphae), and wash the mycelium with sterile water 3-4 times. Subsequently, sterilized tap water was added to the plate containing the mycelia, and continued to be placed in a constant temperature incubator at 25°C for overnight culture in the dark. ...

Embodiment 3

[0024] Embodiment 3: Preparation of a spray whose active ingredient is aceteridin.

[0025] In terms of weight, mix teretin and the auxiliary material detergent 1:1, stir continuously until uniform, and dilute 100-1000 times with water when spraying. On the one hand, the auxiliary material can improve the surface activity, solubility and permeability of the medicament, and it is easy to clean, so that acetaminophen can better act on the oomycete and improve the control effect.

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Abstract

The invention belongs to the field of biological prevention and treatment of plant diseases, and discloses application of a plant-derived compound aesculetin in prevention and treatment of plant oomycete diseases for the first time. Tests prove that under the in-vitro condition, the aesculin has a very strong inhibition effect on hypha growth and spore germination of phytophthora capsici, phytophthora sojae, phytophthora infestans, phytophthora nicotianae, pythium aphanidermatum, pythium ultimum and grape downy mildew. Meanwhile, living body test results also show that aesculetin has a good prevention and treatment effect on pepper phytophthora blight, rape damping-off and grape downy mildew caused by the plant pathogenic oomycetes, and can be used for preventing and treating oomycetes diseases. The method has the advantages: 1, the aesculetin is used for preventing and treating oomycete diseases, so that the pollution to the environment is small, the residue on crops is low, and the method is relatively safe to non-targets; 2, the aesculetin is used for preventing and treating oomycete diseases, so that the use amount of chemical pesticides can be reduced, and the problem of drugresistance is relieved; 3, the aesculetin is rich in source, so that the cost for developing a prevention and treatment medicament taking aesculetin as a main component is relatively low;

Description

technical field [0001] The invention relates to the application of a botanical compound auretin in the prevention and treatment of plant oomycete diseases, and belongs to the field of biological control of plant diseases. Background technique [0002] Plant pathogenic oomycetes are an important class of pathogens that threaten global agricultural production, such as Phytophthora infestans ( Phytophthora infestans ), Phytophthora capsici ( Phytophthora capsici ), Pythium melon and fruit that cause damping-off of rapeseed ( Pythium aphanidermatum ) and the grape downy mildew that causes grape downy mildew ( Plasmopara viticola )Wait. Due to the lack of resistant varieties, chemical control is still the main measure for the prevention and control of plant oomycete diseases. Basically ineffective against oomycetes. At present, production mainly relies on chemical fungicides such as metalaxyl, azoxystrobin, and dimethomorph to prevent and control oomycete diseases, but wi...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00
CPCA01N43/16
Inventor 王碧赵兴增徐曙冯煦单宇陈雨王奇志管福琴刘飞印敏
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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