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Application of Ag nanoparticles to preparation of medicines for restraining nosema bombycis as main pathogen of bombyx mori

A technology of silkworm micro-particles and nano-silver, which is applied in the field of sericulture production, can solve the problems of no definite reports and achieve a significant inhibitory effect

Inactive Publication Date: 2020-08-28
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no definite report on whether silkworm microparticles, one of the main pathogenic microorganisms of the silkworm, also have a broad-spectrum disinfection effect.

Method used

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  • Application of Ag nanoparticles to preparation of medicines for restraining nosema bombycis as main pathogen of bombyx mori
  • Application of Ag nanoparticles to preparation of medicines for restraining nosema bombycis as main pathogen of bombyx mori
  • Application of Ag nanoparticles to preparation of medicines for restraining nosema bombycis as main pathogen of bombyx mori

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the preparation of nano silver

[0026] Preparation of nano-silver: Add silver nitrate to a beaker of ultrapure water at 1% by silver nitrate solution mass fraction, then add 1g bovine serum albumin, place the beaker in a boiling water bath and stir constantly, and adjust the pH by NaOH solution to about 10, control the final volume to 50ml, and react for 30min to 60min to stabilize the nano-silver and obtain a nano-silver concentration of 100μg / mL.

Embodiment 2

[0027] Embodiment 2, nano-silver characterization

[0028] Because the physicochemical properties and biological properties of silver nanoparticles can change along with their size and shape, their size and shape are generally considered to be very important. Through the transmission electron microscope observation, the size and distribution of the used material nano-silver were characterized and analyzed ( figure 1 ), transmission electron microscope images represent different magnifications of silver nanoparticles. The results show that nano-silver is spherical, with a size range of approximately 3-24nm, of which the main distribution is at 3-18nm, and the lattice stripes of nano-silver are measured, and its lattice spacing is 0.23nm, which is the plane distance from silver. consistent. The analysis results show that the nano-silver exists in the form of metal, not in the form of silver compound.

Embodiment 3

[0029] Embodiment 3, the influence of nano-silver on the growth and development of silkworm

[0030] In order to further detect the effect of nano-silver on the growth and development of silkworm larvae, five concentrations of nano-silver, 1 μg / mL, 10 μg / mL, 25 μg / mL, 50 μg / mL and 100 μg / mL were selected in this study, and the mulberry leaves were soaked every day. , the mulberry leaves soaked in ultrapure water were used as the control group, and the silkworms began to add food from the 5th instar until they went up to the stack. The results showed that after 1 μg / mL, 10 μg / mL, 25 μg / mL, 50 μg / mL and 100 μg / mL nano-silver treatment, there was no significant difference in the body weight of silkworm larvae between each treatment group and the control, indicating that the concentrations below 100 μg / mL Nanosilver had no effect on the body weight of silkworm larvae ( figure 2 ).

[0031] In order to further detect the effect of nano-silver on the economic traits and egg-layin...

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Abstract

The invention discloses an application of Ag nanoparticles to preparation of medicines for restraining nosema bombycis as a main pathogen of bombyx mori. The Ag nanoparticles are spherical, are 3-24nmin dimension and are 0.23nm in crystal lattice distance. Through Ag nanoparticle solution of different concentrations, the toxicity of bombyx mori larvae is tested, and the result shows that the weight, the cocoon shell rate and the oviposition number of the bombyx mori larvae between groups do not have significant differences, which indicates that the Ag nanoparticle solution having the concentration being 100 [mu]g / mL or below is safe to the bombyx mori larvae; a mechanism of Ag nanoparticles for resisting nosema bombycis is analyzed, then the inventor finds that the Ag nanoparticles can seriously destroy the structure of the nosema bombycis, when the Ag nanoparticles act with spore coats, the permeability is increased, so that some substances of DNA, proteins and the like are destroyed, the infestation capacity is greatly reduced, the pathogenic capacity of the nosema bombycis is reduced, and the disinfection effects of the nosema bombycis can be achieved; and the Ag nanoparticleshave important significance in controlling the nosema bombycis of the bombyx mori.

Description

technical field [0001] The invention relates to the technical field of sericulture production, in particular to a drug capable of inhibiting silkworm microparticles in sericulture production. Background technique [0002] Silkworm microparticles (Nosema bombycis, N.b) can infect silkworms (Bombyx mori) and cause silkworm microparticle disease (Pèbrine), causing significant economic losses to sericulture. The current methods of preventing and controlling silkworm microparticles mainly include: microscopic examination of female moths to eliminate toxic silkworm eggs, strict disinfection treatment during breeding, and treatment with chemical drugs such as Fangweiling and albendazole after infection. Due to the special structure of silkworm microparticles, the effect of existing therapeutic drugs is not stable, and the prevention and control of silkworm microparticles has always been mainly prevention. However, the existing preventive measures are difficult to achieve an ideal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/38A61P33/02
CPCA61K33/38A61P33/02
Inventor 潘敏慧董战旗龙江琼郑宁陈鹏鲁成
Owner SOUTHWEST UNIVERSITY
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