Composition and method for reprogramming human astrocytes into neurons or brain-like organs

A technology of astrocytes and neurons, applied in chemical instruments and methods, biochemical equipment and methods, cells modified by introducing foreign genetic material, etc. It has problems such as self-assembly and inability to obtain a large number of target nerve cells

Active Publication Date: 2020-09-08
FUDAN UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the technology of Pei Gang's research group as mentioned above is the technology disclosed in Chinese patent CN111073855A, which can reprogram human astrocytes into neurons, there are still some defects in the application of this reprogramming method to central nervous tissue regeneration, such as : (1) Astrocytes are directly reprogrammed into post-mi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition and method for reprogramming human astrocytes into neurons or brain-like organs
  • Composition and method for reprogramming human astrocytes into neurons or brain-like organs
  • Composition and method for reprogramming human astrocytes into neurons or brain-like organs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] The present invention first screens out a combination of a pair of genes and four small molecule drugs that can efficiently reprogram human astrocytes into neurons.

[0131] Post-mitotic neurons produced by direct reprogramming of primary glial cells or fibroblasts cannot proliferate in vitro and in vivo. In order to obtain a large number of neuron cells and 3D brain organoids, the key cell reprogramming factor OCT4 was considered in the research process of this application Overexpression can promote the reprogramming process of human astrocytes, while knocking down the cell cycle regulator p53 can promote the proliferation of intermediate reprogrammed cells.

[0132] By simultaneously overexpressing OCT4 and knocking down p53 in primary human astrocytes, (this application will be referred to as "Op53" in the following description), although in the research of this application, astrocytes have been Efficient overexpression of OCT4 and knockdown of p53, but only a few ce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biology, and relates to a composition and method for reprogramming human astrocytes into neurons or brain-like organs. The composition for efficiently reprogramming human astrocytes into neurons comprises a substance capable of realizing OCT4 overexpression, a substance knocking down a cell cycle regulation factor p53, and a small molecule drug capable of inducing human astrocytes to be reprogrammed into neurons, wherein the small molecule drug capable of inducing the human astrocytes to be reprogrammed into the neurons is selected from one or more of the following four small molecule drugs: CHIR99021, SB431542, RepSox or Y27632. Compared with the prior art, the composition and method have the following advantages: regulation and control of two genes are combined; a brand-new small molecular medicine composition capable of efficiently reprogramming human astrocytes into neurons and brain-like organs is obtained through a large amount of screening; and reliability of the system obtained through screening is verified on astrocytes differentiated from human embryonic stem cells H9 and astrocytes of para-tumor tissues of glioma patients.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a composition and method for reprogramming human astrocytes into neurons or brain organoids. Background technique [0002] In 2017, Stem Cell Reports published online the research paper "Direct Generation of Human Neuronal Cells from Adult Astrocytes by Small Molecules" by Pei Gang's research group at the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, reporting the use of a combination of small molecule compounds to achieve the A study of the direct transformation of astrocytes into nerve cells. [0003] Academician Pei Gang's research group used six small molecule drugs to reprogram human astrocytes into functional neurons, figure 1 A diagram of the technical scheme. The specific scheme is: inoculate human adult astrocytes in astrocyte growth medium (AM) pretreated with poly-lysine. Two days later, the cells reached 90% density, and the growt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/10C12N5/079C12N5/0793
CPCC12N5/0619C07K14/4746C07K14/4702C12N5/0618C12N5/0622C12N2510/00C12N2501/603C12N2513/00C12N2501/727
Inventor 邵志成方诗许锦洪
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap