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Electrochemical sensor for blood enzyme detection

An electrochemical and sensor technology, applied in the field of nanomaterial sensors, can solve the problems of affecting the detection effect, poor connection effect, small sample size, etc., and achieve the effects of novel structure, low processing cost, and good detection effect.

Active Publication Date: 2020-09-08
王龙喜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current detection, because the concentration of ALT in the actual detection is generally relatively low, and the sample size is small, the detection accuracy is not good due to the poor combination of the electrode and the sample in the actual detection.
[0004] The electrode modification technology developed in recent years has a very good effect of modifying nanomaterials on the electrodes to improve the accuracy of detection. However, directly modifying nanomaterials on the electrodes leads to poor connection effect and affects the detection effect.

Method used

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  • Electrochemical sensor for blood enzyme detection
  • Electrochemical sensor for blood enzyme detection
  • Electrochemical sensor for blood enzyme detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The blood enzyme tested was alanine aminotransferase (ALT).

[0029] An electrochemical sensor for blood enzyme detection, comprising a base layer 1, a cover layer 5, a microchannel layer 3, an upper electrode layer 4 and a lower electrode layer 2;

[0030] The electrochemical sensor is stacked according to the order of the base layer 1, the upper electrode layer 4, the microchannel layer 3, the lower electrode layer 2, and the covering layer 5 from bottom to top; First through hole 31; Upper electrode layer 4 is provided with counter electrode 41 and reference electrode 42, and lower electrode layer 2 is provided with working electrode 21, the position of reference electrode 42, counter electrode 41, working electrode 21 and microchannel layer The through holes of 3 correspond to each other; the upper electrode layer 4 and the lower electrode layer 2 are provided with lead-out wires leading out the electrodes; The through hole communicates through the second through h...

Embodiment 2

[0041] ALT detection was performed using the sensor of Example 1.

[0042] The principle of the reaction is:

[0043]

[0044] L-glutamic acid + H 2 O+glutamate oxidase (FAD)→α-ketoglutarate+NH 3 + Glutamate oxidase (FADH 2 )

[0045] Glutamate oxidase (FADH 2 )+O 2 →Glutamate oxidase (FAD)+H 2 o 2

[0046] h 2 o 2 →2H + +O 2 +4e -

[0047] The detection method is to add a reaction reagent into the sample hole, the reaction reagent is human serum, and the ALT content in it is 10g / L, 20g / L, 100g / L, 200g / L, 1000g / L; the reaction reagent also contains L - Alanine and alpha-ketoglutarate

[0048] Then, a voltage of 300mv is selected, and the voltage of the working electrode relative to the reference electrode is positive 300mv, and the content of alanine aminotransferase in the sample is measured at room temperature. Each concentration was measured 10 times to get the average value, and a standard regression curve was drawn.

[0049] The measurement results are...

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Abstract

The invention relates to an electrochemical sensor for blood enzyme detection. A glassy carbon plate is directly used as an independent working electrode and is separated from a counter electrode anda reference electrode, so that the processing cost is low, the structure is novel, and the detection effect is good; the counter electrode and the reference electrode are prepared on the same material, and a platinum electrode is fully utilized to prepare the reference electrode when the counter electrode and the reference electrode are prepared, so that the manufacturing efficiency can be improved; silver is sprayed on the glassy carbon plate on line, then gold is replaced, and structures such as gold nano dendrites, nano stars or gold nano stars further grow on the basis, so that the connection strength of the modified gold and the glassy carbon plate is greatly improved, the specific surface area is increased, and the detection precision is improved. The prepared nanostructure can be adjusted, so that the relationship between the prepared morphology and the detection sensitivity can be further fully researched.

Description

technical field [0001] The invention relates to the field of nanomaterial sensors, in particular to an electrochemical sensor for blood enzyme detection. Background technique [0002] Alanine aminotransferase (ALT) mainly exists in various cells, especially in liver cells. The content of transaminase in the whole liver is about 100 times that in blood. Normally, as long as a small amount is released into the blood, the enzyme activity in the serum can be significantly increased. In the acute stage of various viral hepatitis and drug poisoning liver cell necrosis, ALT is released into the blood in large quantities, so it is an important indicator for diagnosing viral hepatitis and toxic hepatitis. The concentration of alanine aminotransferase in liver cells is 1000-3000 times higher than that in serum. As long as 1% of liver cells are necrotic, the activity of enzymes in the blood can be doubled, so transaminases (especially ALT) are sensitive signs of acute liver cell dama...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/26
CPCG01N27/30G01N27/3272G01N27/26
Inventor 王龙喜
Owner 王龙喜