Novel coronavirus vero cell inactivated vaccine virus solution and production method thereof

A coronavirus and production method technology, applied in the field of novel coronavirus Vero cell inactivated vaccine virus liquid, can solve the problems of no novel coronavirus liquid technical report, no effective preventive vaccine for novel coronavirus, etc., and achieve batch-to-batch differences Highly controllable, uniform quality, high cell seeding density
CN111662881BActive Publication Date: 2021-06-18BEIJING BIOLOGICAL PROD INST CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING BIOLOGICAL PROD INST CO LTD
Publication Date
2021-06-18

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Abstract

The invention provides a novel coronavirus Vero cell inactivated vaccine virus solution and a production method thereof. The method comprises: adding cell growth liquid to a basket bioreactor filled with sheet-like carriers, inoculating Vero cells for culture, and then washing with cell maintenance liquid after growing into a dense monolayer; injecting cells to maintain The MOI of the virus inoculation is 0.005-0.4, the virus culture temperature is 36±1°C, the dissolved oxygen is ≥40%, and the pH value of the virus culture stage is controlled to be 7.2-7.6; after the virus inoculation, cultivate for 48 ~96h, the virus fluid was harvested. In the present invention, the novel coronavirus Vero cell inactivated vaccine virus liquid is successfully cultivated by using a basket reactor. The present invention has high cell inoculation density, high virus titer, highly controllable batch-to-batch difference, and uniform quality; it is a new type of coronavirus inactivated The large-scale production of vaccines has laid a solid foundation.
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Description

technical field

[0001] The invention relates to a novel coronavirus Vero cell inactivated vaccine virus liquid and a production method thereof, specifically a method for producing a novel coronavirus inactivated vaccine virus liquid using a basket bioreactor using Vero cells as a matrix, and utilizing The method produces the novel coronavirus Vero cell inactivated vaccine virus liquid. Background technique

[0002] A new type of coronavirus appeared in 2019, which was confirmed by the World Health Organization (WHO) and named as SARS-CoV-2, and the pneumonia caused by this pathogenic infection is called new type of coronavirus pneumonia. At present, there is no effective drug for the treatment of new coronary pneumonia, and the development of a vaccine for new coronary pneumonia is imminent.

[0003] SARS-CoV-2 is a new type of coronavirus with an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. The new coronavirus is another c...

Claims

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