Preparation method of composite enzymolysis-fermentation coupled fermented pine pollen extract

A compound enzymatic hydrolysis and pine pollen technology, applied in the direction of bacteria used in food preparation, food science, application, etc., can solve the problems of pine pollen easy to oxidize storage time, inedible pine pollen, difficulty in digesting pine pollen, etc., and achieve improvement Antioxidant effect, contribution to fermentation efficiency, and effect of improving equipment utilization

Pending Publication Date: 2020-09-18
YANTAI NEW ERA HEALTH IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual production, the problem of short storage time due to the easy oxidation of pine pollen after breaking the wall is often encountered, or the problem that some people have difficulty in digesting pine pollen and seldom use it, or they cannot eat pine pollen because of allergies Product problems, its products cannot satisfy some people

Method used

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  • Preparation method of composite enzymolysis-fermentation coupled fermented pine pollen extract
  • Preparation method of composite enzymolysis-fermentation coupled fermented pine pollen extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) 9000rpm 9000rpm ultrafine grinding of pine pollen for 15 minutes to obtain broken wall pine pollen, and the measured wall breaking rate is 85%;

[0054] The broken wall pine pollen is dropped into fermenter tank, add water in tank by 9 times of dropping into broken wall pine pollen quality, open and stir; Drop into the acid cellulase of 0.1wt% and 0.1wt% by broken wall pine pollen quality meter in fermenter tank % acid pectinase, adjust the pH to 5.0 with hydrochloric acid or sodium hydroxide; heat up to 50°C and keep it warm, enzymolysis treatment for 2h;

[0055] (2) After the enzymolysis is completed, add glucose to the tank in an amount of 0.1 times the mass of broken pine pollen; add peptone in an amount of 0.05 times the mass of broken pine pollen; add whey protein powder in an amount of broken pine pollen 0.025 times the mass; adjust the pH to 7.5 with sodium hydroxide; sterilize at 85°C for 30 minutes;

[0056] (3) After the sterilization, the fermenter was c...

Embodiment 2

[0061] (1) 9000rpm 9000rpm ultrafine grinding of pine pollen for 15 minutes to obtain broken wall pine pollen, and the measured wall breaking rate is 85%;

[0062] The broken-walled pine pollen is dropped into fermenter, and water is added to the tank by 12 times of the quality of broken-walled pine pollen, and stirring is started; in the fermenter, 0.15wt% acid cellulase and 0.15wt% acid cellulase are dropped into the broken-walled pine pollen mass. % acid pectinase, adjust the pH to 5.0 with hydrochloric acid or sodium hydroxide; heat up to 55°C and keep it warm, enzymolysis treatment for 2h;

[0063] (2) After the enzymolysis is completed, add glucose to the tank in an amount of 0.08 times the mass of broken pine pollen; add peptone, and use an amount of 0.04 times the mass of broken pine pollen; add whey protein powder, and use an amount of broken pine pollen 0.015 times the mass; adjust the pH to 7.5 with sodium hydroxide; sterilize at 85°C for 30 minutes;

[0064] (3) A...

Embodiment 3

[0069] (1) 9000rpm 9000rpm ultrafine grinding of pine pollen for 15 minutes to obtain broken wall pine pollen, and the measured wall breaking rate is 85%;

[0070] The broken wall pine pollen is dropped into fermenter tank, add water in the tank by 7 times of dropping into broken wall pine pollen quality, open and stir; Drop into the acid cellulase of 0.08wt% and 0.08wt% by broken wall pine pollen mass meter in fermenter tank % acid pectinase, adjust the pH to 5.0 with hydrochloric acid or sodium hydroxide; heat up to 50°C and keep it warm, enzymolysis treatment for 2h;

[0071] (2) After the enzymolysis is completed, add glucose to the tank in an amount of 0.1 times the mass of broken pine pollen; add peptone in an amount of 0.05 times the mass of broken pine pollen; add whey protein powder in an amount of broken pine pollen 0.025 times the mass; adjust the pH to 7.5 with sodium hydroxide; sterilize at 85°C for 30 minutes;

[0072] (3) After the sterilization is finished, th...

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Abstract

The invention discloses a preparation method of composite enzymolysis-fermentation coupled fermented pine pollen extract. The method comprises the following steps of (1) mixing wall-broken pine pollenwith water, and carrying out enzymolysis treatment by using cellulase and pectinase; (2) adjusting a fermentation environment and performing sterilization; (3) inoculating strains, and performing fermentation for 5-7 days; (4) separating a fermentation liquor obtained in the step (3) to obtain supernate and precipitate; (5) carrying out hot reflux extraction on the precipitate obtained in the step (4), and performing separation to obtain supernate; and (6) combining the supernate obtained in the step (4) and the supernate obtained in the step (5), and performing concentration and drying to obtain the fermented pine pollen extract. According to the process, the yield of the fermented pine pollen extract can be remarkably increased, the sensitization problem of a pine pollen product is solved, and the antioxidant effect of the pine pollen product is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a fermented pine pollen extract coupled by compound enzymolysis-fermentation. Background technique [0002] The method for existing fermented pine pollen is usually to directly ferment in fermenter after the pine pollen is crushed, such as publication number is CN110037271A, title is called " a kind of preparation method of fermented pine pollen ", publication number is CN109588598A " a kind of pollen double Mycobacterium fermentation method and application of its fermentation product", publication number is CN103141732A "A new process for fermenting pine pollen with probiotics", publication number is CN109806190A "a fermentation and extraction process of pine pollen", publication number is CN104938965B Chinese patent application for "Fermentation method with Bacillus coagulans and its fermentation product and application". However, in actual produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L5/20A23L5/00
CPCA23L33/00A23L5/25A23L5/00A23V2002/00A23V2400/165A23V2400/175A23V2400/169A23V2200/30A23V2250/61
Inventor 刘其耸衣洁菡徐宏楠
Owner YANTAI NEW ERA HEALTH IND
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