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Bermudagrass transcription factor cdwrky50 regulating plant salt tolerance and its application

A transcription factor and salt tolerance technology, applied in the field of genetic engineering, can solve problems such as the lack of salt tolerance WRKY transcription factor

Active Publication Date: 2022-03-08
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on the WRKY transcription factors related to salt tolerance in bermudagrass

Method used

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  • Bermudagrass transcription factor cdwrky50 regulating plant salt tolerance and its application
  • Bermudagrass transcription factor cdwrky50 regulating plant salt tolerance and its application
  • Bermudagrass transcription factor cdwrky50 regulating plant salt tolerance and its application

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Embodiment Construction

[0040] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0041] 1. Plant material: the plant material used in the present invention is wild bermudagrass germplasm 'WBG128'.

[0042] 2. Cloning of bermudagrass WRKY50:

[0043] 2.1. Extraction of total RNA from Bermudagrass leaves: take an appropriate amount of Bermudagrass leaves, add liquid nitrogen to grind into powder, transfer to an RNAase-free centrifuge tube, add 1mL Trizol, vortex mix, place at room temperature for 5min, and centrifuge at 4°C and 12000rpm for 5min ; Transfer the supernatant to a new centrifuge tube, add 200 μL of chloroform, mix thoroughly, place at room temperature for 10 min, and centrifuge at 12,000 rpm for 20 min at 4°C; transfer the supernatant to a new centrifuge tube, add an equal volume of isopropyl Alcohol, let stand at room temperature for 10 min, centrifuge at 4°C, 12,000 rpm for 10 min; discard the supernatant, add 1 mL of 75% room temperature ethanol to was...

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Abstract

The invention discloses a bermudagrass transcription factor CdWRKY50 for regulating the salt tolerance of plants and an application thereof, which relates to the field of genetic engineering. The nucleotide sequence of Bermudagrass transcription factor CdWRKY50 is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2. The application of Bermudagrass transcription factor CdWRKY50 is: ① the application of transgenic Arabidopsis with reduced salt tolerance; ② the application of transgenic Arabidopsis with improved salt tolerance through VIGS technology CdWRKY50 Application to silence bermudagrass. For the first time, the present invention cloned and obtained the Bermudagrass transcription factor CdWRKY50, which regulates plant salt tolerance, and found overexpression in functional verification CdWRKY50 Reduced salt tolerance in transgenic Arabidopsis, whereas silencing by VIGS CdWRKY50 The salt tolerance of Bermudagrass has been improved, indicating that the gene negatively regulates the salt tolerance of plants; the present invention CdWRKY50 It provides a new genetic resource for the rapid and precise cultivation of salt-tolerant bermudagrass germplasm through genetic engineering technology.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a bermudagrass transcription factor CdWRKY50 regulating plant salt tolerance and application thereof. Background technique [0002] Salt stress is one of the main abiotic stresses affecting plant growth and grain production. According to statistics, the global salinized soil area is about 8.3×10 8 hectares, accounting for 6% of the land area (Rengasamy, 2016). Among them, the salinized soil area in my country is as high as 36 million hectares, accounting for 4.88% of the national usable area (Wang Jiali et al., 2011), which seriously restricts the development of my country's economy and society. Bermudagrass (Cynodondactylon) is a perennial herb belonging to the Poaceae Bermudagrass genus. It has the characteristics of fine texture, dark green color and strong rhizome spread. It is widely used in gardens, parks, football fields, golf courses, etc. It is the main warm season ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00A01H6/46A01H6/20
CPCC07K14/415C12N15/8273C12N15/8218
Inventor 陈良黄雪冰曹丽雯马光婧
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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