Plant salt tolerance related protein as well as related biological material and application thereof

Abstract

The invention discloses a plant salt tolerance related protein as well as a related biological material and application thereof, and provides a salt tolerance related protein derived from rice, which is named as OsELF4a and derived from rice Nipponbare. An experiment for inhibiting the content of the protein in a target plant by utilizing a CRISPR / Cas9 gene editing system shows that compared with the Nipponbare wild type rice, the survival rate of osef4a-1 rice with the function of OsELF4a lost after high-salt hypochondriac stress culture is 30% and is far lower than the survival rate of 70% of the Nipponbare rice, that is, the salt tolerance of the rice can be reduced due to the loss of the function of OsELF4a, which is shown that the OsELF4a is a protein related to the rice tolerance, and specifically, the salt tolerance of the rice without the OsELF4a function can be reduced.

Description

technical field [0001] The invention relates to a plant salt-tolerance-related protein in the field of biotechnology, as well as related biological materials and applications. Background technique [0002] The problem of land salinization is increasing year by year worldwide, causing massive losses to agricultural production, and is a major obstacle to sustainable agricultural development (Tester and Davenport 2003). The salinity in the soil mainly comes from irrigation water and seawater intrusion caused by earthquakes and tsunamis, which contains a large amount of NaCl, which poses a serious threat to crop growth (Deinlein et al.2014; Takagi et al.2015). Plants are divided into tolerant and sensitive according to the tolerance of crops to salt stress. Tolerant crops can survive in lands with a salt concentration exceeding 200m3M, while sensitive crops are difficult to survive in lands with a salt concentration of Survive in the land of 100mM-200mM. Studies have found tha...

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Problems solved by technology

(2) Ion poisoning is mainly due to the high concentration of Na + and Cl - The balance of sodium and potassium ions is very important for the metabolism of plants. High concentrations of NaCl cause disturbances in the absorption of ions in plant roots, including K + The reduction of absorption and metabolic disturbance will also cause the reduction of photosynthetic efficiency (Deinlein et al.2014; Yang and Guo 2018)

(2) Ion transport, the maintenance of intracellular ion homeostasis is very important for plants to resist salt. Na in cells after salt treatment + Concentration increases to reduce cell damage and deplete harmful Na + Efflux, cells mainly maintain the balance of sodium and potassium ions in the cell through three ways: 1. Through Na + The antiporter SOS1 converts Na + Efflux, the circadian clock GI is involved in rice salt resistance, the interaction between GI and SOS2 inhibits the interaction between SOS2 and SOS3, thereby inhibiting the phosphorylation of SOS1 by SOS2, and the GI protein will be degraded after salt treatment, so that the interaction between SOS2 and SOS3 phosphorylates SOS1 , SOS1 acts as an antiporter to reduce intracellular Na + content (Kim et al.2013); 2. Reduce the intake of sodium ions and increase the intake of potassium ions through the sodium potassium transporter. HKTs family genes are involved in the transport of sodium and potassium ions. Recent studies have found that OsHKT1.5( SKC1) is involved in rice salt resistance. Under salt stress, OsHKT1.5 can effectively transport Na+ from the stem to the root, improving the salt tolerance of rice (Ren et al.2005); 3. Through NHX and other sodium potassium transporters Sodium ion compartmentalization (Yang and Guo 2018), previous studies have found that NHX1 can effectively compartmentalize Na+ in vacuoles, but recent studies have found that NHX is involved in the dual transport of sodium and potassium, and its mutants are not very good Salt resistance (Bassil et al. 2011)

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