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A laccase-nanoflower-γ-fe 2 o 3 Copper alginate gel beads and preparation method and use thereof

A technology of copper alginate and -fe2o3, applied in biochemical equipment and methods, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problem of high synthesis cost, complex synthesis process and low specific activity of mesoporous materials Low-level problems, to achieve good operational stability, simple preparation process, and good stability

Active Publication Date: 2022-05-03
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, in the synthesis of mesoporous silica microspheres, block copolymers (P123) need to be used as templates to mediate the appearance of mesoporous structures, resulting in higher synthesis costs of mesoporous materials, and high temperature calcination is required after synthesis. Removing templates not only consumes energy but also requires equipment such as muffle furnaces and reactors, which makes the synthesis process relatively complicated
[0008] In addition, using Fe 3 o 4 @SiO 2 -NH 2 or Fe 2 o 3 @MSS-NH 2 When used as a carrier, the amino group on the laccase is covalently linked to the amino group on the surface of the carrier through the aldehyde group on the bifunctional cross-linking reagent. less active than free enzyme

Method used

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  • A laccase-nanoflower-γ-fe <sub>2</sub> o <sub>3</sub> Copper alginate gel beads and preparation method and use thereof
  • A laccase-nanoflower-γ-fe <sub>2</sub> o <sub>3</sub> Copper alginate gel beads and preparation method and use thereof
  • A laccase-nanoflower-γ-fe <sub>2</sub> o <sub>3</sub> Copper alginate gel beads and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A Laccase-Nanoflower-γ-Fe 2 o 3 The preparation method of copper alginate gel beads, comprising:

[0039] (1) Synthesis of laccase-copper phosphate hybrid nanoflowers

[0040] Use phosphate buffer (pH7.0, 0.05M) to prepare 3000mL of laccase solution with a concentration of 0.1mg / mL, and then add 20mL of CuSO 4 Solution (120mM), the above mixture was left at room temperature for 72h, and the precipitate was separated from the solution by centrifugation to obtain laccase-copper phosphate hybrid nanoflowers.

[0041] (2) γ-Fe 2 o 3 Synthesis

[0042] Ferric chloride (200mL, 0.4M) and ferrous chloride (200mL, 0.5M) were added to ammonia solution (200mL, 0.2M), and the precipitate was separated from the solution by centrifugation. In order to obtain an acidic sol, the precipitate was added to Stir in 0.25M perchloric acid aqueous solution for flocculation, so that Fe 3 o 4 The particles are aggregated and precipitated, and then oxidized by ferric nitrate at 90°C, and...

Embodiment 2

[0048] A Laccase-Nanoflower-γ-Fe 2 o 3 The preparation method of copper alginate gel beads is basically the same as the preparation method of Example 1, the difference is: in the laccase-nanoflower-γ-Fe 2 o 3 During the synthesis of copper alginate gel beads, the amount of laccase-copper phosphate hybrid nanoflowers used was 25 mg.

Embodiment 3

[0050] A Laccase-Nanoflower-γ-Fe 2 o 3 The preparation method of copper alginate gel beads is basically the same as the preparation method of Example 1, the difference is: in the laccase-nanoflower-γ-Fe 2 o 3 During the synthesis of copper alginate gel beads, the amount of laccase-copper phosphate hybrid nanoflowers used was 10 mg.

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Abstract

The invention discloses a laccase-nanoflower-γ-Fe 2 o 3 Copper alginate gel beads and its preparation method and application, the gel beads are passed through sodium alginate, γ-Fe 2 o 3 Ferrofluid colloidal solution and laccase-copper phosphate hybrid nanoflowers were stirred in water and added dropwise to copper chloride solution at a constant speed; sodium alginate, γ-Fe 2 o 3 The mass ratio of ferrofluid colloid solution to laccase-copper phosphate hybrid nanoflowers is 3000:30000-70000:5-50; γ-Fe 2 o 3 In ferrofluid colloidal solution, γ‑Fe 2 o 3 The particle diameter of the particle is 10nm; the diameter of the laccase-copper phosphate hybrid nanoflower is 15-20 μm. Laccase-nanoflower-γ-Fe of the present invention 2 o 3 Copper alginate gel beads showed higher catalytic efficiency than free enzymes in catalyzing the degradation of chlorophenols and the oxidation of polycyclic aromatic hydrocarbons and phenolic compounds, and showed good reusability and storage stability.

Description

technical field [0001] The invention relates to a copper alginate gel bead, in particular to a laccase-nano flower-γ-Fe 2 o 3 Copper alginate gel beads, preparation method and application thereof. Background technique [0002] Chlorophenols are toxic. As the number of chloride ions increases, their toxicity increases, and they are more difficult to be oxidized. Chlorophenols are difficult to be degraded by microorganisms and are serious pollutants. 2. 4-dichlorophenol, 4-p-chlorophenol and 2-o-chlorophenol have a strong denaturing effect on organisms, are irritating to the skin and mucous membranes, and are corrosive, seriously endangering health. [0003] Polycyclic aromatic hydrocarbons are a class of hydrocarbons containing two or more benzene rings. Studies have shown that various polycyclic aromatic hydrocarbons are carcinogenic, mutagenic and reproductively toxic. PAHs are mainly produced by incomplete combustion of organic materials, such as factory exhaust gas, v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/34C02F101/34C02F101/36C02F101/32
CPCC12N11/14C12N11/10C12N11/04C12N9/0061C02F3/342C12Y110/03002C02F2101/345C02F2101/36C02F2101/327
Inventor 吴卓夫王君吕海艳张斯童于潇潇
Owner JILIN AGRICULTURAL UNIV
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