Primer and test kit for detecting serum exosome CTD-2376I4-1 and application of primer in diagnosis and treatment of gastric cancer with liver metastasis

A technology of exosomes and kits, applied in the field of molecular diagnosis

Pending Publication Date: 2020-09-22
ZHENJIANG NO 1 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported that CD44v6, OPN, YB-1, MAGE-A10 and other molecules may be used as po

Method used

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  • Primer and test kit for detecting serum exosome CTD-2376I4-1 and application of primer in diagnosis and treatment of gastric cancer with liver metastasis
  • Primer and test kit for detecting serum exosome CTD-2376I4-1 and application of primer in diagnosis and treatment of gastric cancer with liver metastasis
  • Primer and test kit for detecting serum exosome CTD-2376I4-1 and application of primer in diagnosis and treatment of gastric cancer with liver metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Detection of CTD-2376I4-1 in serum exosomes

[0035] 1. Obtain the serum sample to be tested:

[0036] Serum samples from 120 cases of gastric cancer and 120 normal persons.

[0037] The samples are the serum samples of the patients with gastric cancer and normal people with coagulant added.

[0038] 2. Extract exosomes from serum samples to be tested and verify

[0039] According to the instructions of the exosome extraction kit, the exosomes in the gastric serum were extracted, and the steps were as follows: (1) Whole blood was centrifuged at 3000×g for 15 min (centrifugal radius 10 cm) to remove cells or cell debris. (2) Put the upper liquid into a centrifuge tube, add 63 μl ExoQuick reagent to 250 μl serum, put it at 4°C for 30 minutes; (3) Centrifuge the mixture at 1500×g (centrifugal radius 10cm) for 30 minutes at 4°C; ( 4) Aspirate all the supernatant, centrifuge at 1500×g (centrifugal radius 10cm) for 5 min at 4°C, and aspirate the entire supernatan...

Embodiment 2

[0070] Example 2 Clinical significance of serum exosome CTD-2376I4-1

[0071] The correlation between serum exosomal CTD-2376I4-1 expression and clinical parameters such as gender, age, tumor diameter, clinical stage, degree of tissue differentiation, lymph node metastasis, and liver metastasis of gastric cancer was further analyzed. The results found that, as shown in Table 4 and Table 5, the expression level of serum exosomal CTD-2376I4-1 was significantly correlated with the depth of invasion, lymph node metastasis, liver metastasis, and clinical stage of patients (P values ​​were 0.047, 0.036, 0.001, and 0.003, respectively. ), but not significantly correlated with gender, age, tumor location, tumor diameter, tissue differentiation, Helicobacter pylori infection and CEA level. The results of multivariate analysis suggested that the high expression of serum exosomal CTD-2376I4-1 was one of the independent prognostic factors for gastric cancer liver metastasis.

[0072] Tab...

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Abstract

The invention relates to a primer and a test kit for detecting serum exosome CTD-2376I4-1 and application of the primer in diagnosis and treatment of gastric cancer with liver metastasis. The test kitcomprises an RNA extraction reagent of a serum lncRNA sample, the primer, a genome-free DNA reaction system, a reverse transcription reaction system and a qPCR operation reaction system. The invention discloses an expression condition of lncRNA in serum exosomes of patients with gastric cancer and normal people, and CTD-2376I4-1 is detected by using fluorescent quantitative PCR (Polymerase ChainReaction). The serum exosome CTD-2376I4-1 can be used for diagnosing the early gastric cancer with liver metastasis, judging disease progression and prognosis conditions, and evaluating the treatmenteffect of the gastric cancer with liver metastasis.

Description

technical field [0001] The invention relates to primers and a kit for detecting serum exosome CTD-2376I4-1 and its application in the diagnosis and treatment of gastric cancer liver metastasis, belonging to the technical field of molecular diagnosis. Background technique [0002] Gastric cancer is a serious threat to human health and is one of the five major cancers in the world. The World Health Organization statistics report shows that in 2012, there were about 951,000 new cases of gastric cancer worldwide, and about 723,000 deaths. Gastric cancer rates are higher in East Asia (particularly China, Korea, Mongolia, and Japan), Central and Eastern Europe, South America, and most of Africa. China is the country with the highest incidence and mortality rate of gastric cancer in the world. According to data from the National Cancer Registry Center, there were about 679,000 new cases of gastric cancer in China in 2015, and about 498,000 deaths, ranking second among malignant tu...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 范钰何莲郎亚昆谷旭宇周永静龚丹丹
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
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