Application of berberine dimer analog derivatives in the preparation of drugs for preventing and treating Parkinson's disease
A technology for catechin and Parkinson's disease, applied in the field of medicine, can solve the problem of not enough catechin, and achieve the effects of reducing apoptosis, preventing and treating Parkinson's disease, and protecting from oxidation
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Embodiment 1
[0056] The toxicity of embodiment 1 berberine and JJA-D0 to SH-SY5Y cells
[0057] SH-SY5Y cells were divided into 5×10 4 / mL inoculated in 96-well culture plate, 100 μL / well, put into 5% (v / v) CO 2 Cell culture incubator, cultured at 37 ℃ for 24h. Take out, except that the blank control group (Ctrl) is replaced with fresh culture medium, other each groups respectively replace culture medium with the culture medium that contains different concentrations of berberine (Apocynin) and JJA-D0; Wherein, the final concentration of berberine The final concentrations of JJA-D0 were 12.5, 25, 50, 100, 200, 400 μM respectively. Four replicate wells were set up for each group, and after continuing to culture for 24h, 36h, 48h, and 72h respectively, the 96-well culture plate was taken out, and MTT solution (15 μL / well) was added to each well to make the final concentration 0.5 mg / mL. After putting it into the incubator and incubating for 4 hours, suck out the solution in each well, then...
Embodiment 2
[0058] Example 2 MPP + Treatment of SH-SY5Y cells to establish in vitro Parkinson's disease cell model
[0059] SH-SY5Y cells were divided into 5×10 4 / mL inoculated in 96-well culture plate, 100 μL / well, put into 5% (v / v) CO 2 Cell culture incubator, cultured at 37 ℃ for 24h. Take it out, and replace the culture medium with different concentrations of 1-methyl-4-phenyl-pyridinium (MPP+ ) culture medium; wherein, MPP + The final concentrations were 0, 50, 125, 250, 500, 1000, 2000 μM, respectively. After continuing to culture for 24 hours, the 96-well culture plate was taken out, and MTT solution (15 μL / well) was added to each well with a final concentration of 0.5 mg / mL. After incubating for 4 hours in an incubator, suck out the solution in each well, then add DMSO (150 μL / well) to dissolve the crystals, shake for 10 minutes, and detect the OD value with a microplate reader at a detection wavelength of 570 nm. Calculate different MPP + The cell viability at the concentr...
Embodiment 3
[0060] Example 3 Protective effect of berberine on PD model cells
[0061] SH-SY5Y cells were divided into 5×10 4 / mL inoculated in 96-well culture plate, 100 μL / well, put into 5% (v / v) CO 2 In a cell culture incubator, culture at 37°C for 24h. Take out, replace the culture solution of the dosing group with the medium pre-protection 4h containing berberine of different concentrations, wherein, the final concentration of berberine is respectively 500, 1000, 1500 μ M; Blank control group (Ctrl) and model group ( Model) only fresh medium was added. After 4 hours, the medium of the model group and the drug-dosed group were added with MPP + The final concentration was 1 mM, and 4 replicate wells were set up for each group. After continuing to culture for 24 hours, the 96-well culture plate was taken out, and MTT solution (15 μL / well) was added to each well with a final concentration of 0.5 mg / mL. After putting it into the incubator and incubating for 4 hours, suck out the solu...
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