Micro-fluidic chip for obtaining rare cell and being encapsulated with single cells

Abstract

The invention discloses a micro-fluidic chip for obtaining a rare cell and being encapsulated with single cells. The micro-fluidic chip is sequentially provided with an upper flowpath layer, a filtration membrane layer and a lower flowpath layer from top to bottom, wherein a millipore filter technique is adopted for enriching rare cells in sample liquid, and a liquid drop technique is adopted forencapsulating the enriched cells. A dielectrophoresis technique is adopted for refining and sorting. The micro-fluidic chip integrates the functions of cell filtering, backward flushing, unicellular liquid drop encapsulating and dielectrophoresis sorting, so that high purity unicellular automatic encapsulating interation of the rare cells in samples can be realized, and the micro-fluidic chip canbe directly used for subsequent unicellular control and analysis.

Description

technical field [0001] The invention relates to biological sample processing technology, in particular to a microfluidic chip used for rare cell acquisition and single cell encapsulation. Background technique [0002] In the past decade, microfluidic technology for biological sample processing applications has developed rapidly. Microfluidic chips, also known as "lab-on-a-chip", can effectively overcome the disadvantages of traditional medical equipment such as large volume, long reaction time, and high sample consumption, and integrate the preparation, processing, and transmission of biological samples into a tiny chip. , has been widely used in basic research, pathological diagnosis, adjuvant therapy and other fields. In microfluidic chips, various technologies such as bio-affinity technology, microporous membrane filtration, droplet technology, fluid inertial manipulation, dielectrophoretic manipulation, magnetic manipulation, and acoustic wave manipulation are applied t...

AI Technical Summary

Problems solved by technology

[0003] At present, most of the microfluidic chips for rare cell processing are limited to the level of cell sorting, and it is not possible to integrate rare cell sorting and single-cell packaging functions on a single microfluidic chip.

As a result, the application of rare cells is limited to the counting level, and it is difficult to achieve subsequent single-cell manipulation and analysis

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