Composition for preventing and/or treating alcoholic liver injury and application thereof
A technology of alcoholic liver injury and composition, which is applied in the field of biomedicine to achieve good repairing effect, excellent preventive effect, and excellent prevention effect of alcoholic liver injury
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[0037] The invention provides a composition for preventing and / or treating alcoholic liver injury, comprising the following components in parts by mass: 0.8-1.2 parts of wolfberry glycopeptide and 0.8-1.2 parts of olive oil; the preparation method of the wolfberry glycopeptide The method includes: performing subcritical butane extraction on wolfberry powder, collecting the extraction residue, performing subcritical water extraction on the extraction residue, collecting the extract, and collecting and collecting glycopeptides with a molecular weight cut-off between 10kD and 30kD with an ultrafiltration membrane to obtain Lycium barbarum glycopeptide; the content of hydroxytyrosol in the olive oil is 205-215 mg / kg, preferably 210 mg / kg.
[0038] In the present invention, the composition preferably includes the following components in parts by mass: 1 part of wolfberry glycopeptide and 1 part of olive oil.
[0039] In the present invention, the mass percentage of protein in the w...
Embodiment 1
[0080] 1. Preparation of wolfberry glycopeptide:
[0081] 1) Grind wolfberry fruit, pass through a 40-mesh sieve, put it into an extraction bag, add it to a subcritical extraction device, use butane as the extraction medium, feed the wolfberry fruit powder according to the material-solution ratio of 1kg: 1.5L, and the extraction pressure is 0.5MPa. The extraction temperature is 40°C, and the extraction time is 60 minutes. After the extraction is finished, the wolfberry residue is obtained.
[0082] 2) Put the above wolfberry slag into the extraction bag and add it to the subcritical water extraction device. The wolfberry powder is fed according to the ratio of material to liquid: 1kg: 1.5L, the subcritical temperature is 120°C, and the subcritical time is 15min. After the extraction is completed, extract The liquid is directly pumped into the tube centrifuge with a centrifugal speed of 16000rpm.
[0083] 3) Concentrate the wolfberry extract to 2 times the volume of the wolfb...
Embodiment 2
[0099] Example 2 Effect of the composition of Example 1 on the survival rate of alcohol-induced hepatocytes
[0100] L-02 cells in the logarithmic growth phase were seeded in 96-well plates and incubated at 37°C for 24h. Silymarin (SM) was used as a positive control.
[0101] The prevention group had a positive control group SM-Pre (24 μg / mL), and the administration groups were LBP-Pre (24 μg / mL) of the wolfberry glycopeptide group, OO-Pre (24 μg / mL) of the olive oil group and the olive oil of the wolfberry glycopeptide group. Oil (mass ratio 1:1) composition group OO+LBP-Pre (24μg / mL);
[0102] The repair group had a positive control group SM-Post (24 μg / mL), and the administration groups were LBP-Post (24 μg / mL) of the wolfberry glycopeptide group, OO-Post (24 μg / mL) of the olive oil group and the olive oil of the wolfberry glycopeptide group. Oil (mass ratio is 1:1) composition group OO+LBP-Post (total concentration 24μg / mL), 5 duplicate wells in each group, absorb and di...
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