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Method for separating and preparing trehalose from sucrose isomerase enzymatic hydrolysate

A technology of isomerase enzymatic hydrolysis solution and sucrose isomerase, which is applied to the preparation of sugar derivatives, chemical instruments and methods, disaccharides, etc., can solve the problems of low yield and expensive instruments, and achieve high purity and instrumentation The effect of high degree of automation and convenient operation

Active Publication Date: 2020-10-09
李宪臻
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the used instruments of these preparation methods are expensive and the yield is low.
[0006] At present, there is no report on the separation and preparation of trehalulose by HILIC method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The sucrose isomerized by the sucrose isomerase to obtain a sucrose isomerase hydrolyzate, and the sucrose isomerase hydrolyzate contains trehalulose.

[0030] Concentrate 500 mL of the sucrose isomerase hydrolyzate to 25 mL, wherein the reducing sugar concentration in the sucrose isomerase hydrolyzate is 500 mg / mL. First decolorize the concentrated sucrose isomerase hydrolyzate with macroporous resin AB-80, the amount of sample loaded is recorded as reducing sugar mass, which is 0.1% of the mass of macroporous resin, rinse with ethanol with a volume ratio of 10%, Elute with 30% ethanol by volume, and collect the eluate for concentration. The concentrated decolorized sucrose isomerase hydrolyzate is used to remove macromolecular impurities with C18 SPE solid phase extraction column, and some residual pigments can be removed at the same time. The amount of sample loaded is calculated based on the mass of reducing sugar, which is 0.2wt% of the C18 SPE filler. Rinse the S...

Embodiment 2

[0033] After sucrose isomerized by sucrose isomerase to produce sucrose isomerase hydrolyzate, the obtained sucrose isomerase hydrolyzate is further used to produce isomaltulose to obtain waste syrup, which contains trehalulose .

[0034] Concentrate 500 mL of the sucrose isomerase hydrolyzate to 25 mL, wherein the reducing sugar concentration in the sucrose isomerase hydrolyzate is 500 mg / mL. First decolorize the concentrated sucrose isomerase hydrolyzate with macroporous resin D-101, the amount of sample loaded is recorded as reducing sugar mass, which is 10% of the mass of macroporous resin, rinsed with ethanol with a volume ratio of 10%, Elute with 60% ethanol by volume, and collect the eluate for concentration. The concentrated decolorized sucrose isomerase hydrolyzate is used to remove macromolecular impurities with C18 SPE solid phase extraction column, and some residual pigments can be removed at the same time. The amount of sample loaded is measured by the mass of r...

Embodiment 3

[0037] The sucrose isomerized by the sucrose isomerase to obtain a sucrose isomerase hydrolyzate, and the sucrose isomerase hydrolyzate contains trehalulose.

[0038] Concentrate 500 mL of the sucrose isomerase hydrolyzate to 25 mL, wherein the reducing sugar concentration in the sucrose isomerase hydrolyzate is 500 mg / mL. First decolorize the concentrated sucrose isomerase hydrolyzate with macroporous resin AB-8, the amount of sample loaded is recorded by the mass of reducing sugar, which is 5% of the mass of macroporous resin, rinse with ethanol with a volume ratio of 20%, Elute with 60% ethanol by volume, and collect the eluate for concentration. The concentrated decolorized sucrose isomerase hydrolyzate is used to remove macromolecular impurities with C18 SPE solid phase extraction column, and some residual pigments can be removed at the same time. The amount of sample loaded is calculated by reducing sugar concentration, which is 10wt% of the C18 SPE packing. The SPE col...

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Abstract

The invention discloses a method for separating and preparing trehalose from sucrose isomerase enzymatic hydrolysate, and belongs to the field of functional sugar separation and preparation. The method comprises the following steps: decoloring enzymolysis by using resin;then removing macromolecular impurities and part of residual pigments in the sucrose isomerase enzymatic hydrolysate by using a solid-phase extraction technology, finally concentrating the treated enzymatic hydrolysate, and further separating and preparing on a self-made hydrophilic chromatographic column by using a hydrophilicchromatography to obtain the high-purity trehalose with the purity of more than 90%.

Description

technical field [0001] The invention relates to a method for separating and preparing trehalulose from sucrose isomerase enzymolysis solution, and belongs to the field of separation and preparation of functional sugars. Background technique [0002] As a traditional sweetener, sucrose is widely used in the food industry. However, eating too much sucrose can cause obesity or dental caries. With the improvement of people's living standards and the increasing emphasis on health, sucrose, as a commonly used Flavoring agents are no longer suitable for the needs of special populations such as obesity and diabetes. People are increasingly demanding new sweeteners that are non-cariogenic, suitable for special populations, and have special functions (Ravaud, S., Robert, X. , Watzlawick, H., et al. Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization. Journal of Biological Chemistry, 2007, 282, 28126–28136.). [0003] Trehalulo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/04C07H1/06
CPCC07H3/04C07H1/06
Inventor 付冬梅孙玉梅李宪臻
Owner 李宪臻
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