Application of carbenoxolone in preparation of anti-Zika virus medicine

A technology of Zika virus and carbenoxolone is applied in the application field of carbenoxolide in the preparation of anti-Zika virus drugs, which can solve the problem of lack of drugs that effectively inhibit Zika virus, and achieves improvement of brain development retardation phenomenon and resources Rich and convenient effects

Inactive Publication Date: 2020-10-16
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the deficiency of the lack of drugs for effectively inhibiting Zika virus in the prior art, and to provide the application of carbenoxic acid in the preparation of anti-Zika virus drugs

Method used

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  • Application of carbenoxolone in preparation of anti-Zika virus medicine
  • Application of carbenoxolone in preparation of anti-Zika virus medicine
  • Application of carbenoxolone in preparation of anti-Zika virus medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 carbenoxic acid is to the toxicity experiment of A549 cell

[0045] Include the following steps:

[0046] 1. Inoculation of A549 cells: Use DMEM medium containing 5% fetal bovine serum to prepare a single cell suspension, and inoculate 2000-3000 cells per well into a 96-well cell culture plate, with an inoculation volume of 100 μl per well.

[0047] 2. Culture A549 cells: at 37°C, 5% CO 2 Under culture conditions, culture for 1 day.

[0048] 3. Add carbenoxic acid: discard the DMEM medium in each well, add 100 μl to each well and dilute with DMEM medium containing 5% fetal bovine serum to the corresponding concentration (10 μM, 25 μM, 50 μM, 100 μM, 200 μM) 100 μl of DMEM medium containing 5% fetal bovine serum without drugs was added to control wells.

[0049] 4. Coloring: After 24 hours of culture, add 10 μl of MTT solution to each well, and incubate at 37°C, 5% CO 2 Continue to incubate under the culture conditions for 4 hours, then terminate the cul...

Embodiment 2

[0054] Example 2 Toxicity Experiment of Glycyrrhizin to A549 Cells

[0055] Include the following steps:

[0056] 1. Inoculation of A549 cells: Use DMEM medium containing 5% fetal bovine serum to prepare a single cell suspension, and inoculate 2000-3000 cells per well into a 96-well cell culture plate, with an inoculation volume of 100 μl per well.

[0057] 2. Culture A549 cells: at 37°C, 5% CO 2 Under culture conditions, culture for 1 day.

[0058] 3. Add glycyrrhizin: discard the DMEM medium in each well, add 100 μl to each well and dilute to the corresponding concentration (10 μM, 25 μM, 50 μM, 100 μM, 200 μM) with DMEM medium containing 5% fetal bovine serum Glycyrrhizin (GA), 100 μl of drug-free 5% fetal bovine serum DMEM medium was added to the control well.

[0059] 4. Coloring: After 24 hours of culture, add 10 μl of MTT solution to each well, and incubate at 37°C, 5% CO 2 Continue to incubate under the culture conditions for 4 hours, then terminate the culture, di...

Embodiment 318

[0065] Example 3 Toxicity test of 18β-glycyrrhetinic acid on A549 cells

[0066] Include the following steps:

[0067] 1. Inoculation of A549 cells: Use DMEM medium containing 5% fetal bovine serum to prepare a single cell suspension, and inoculate 2000-3000 cells per well into a 96-well cell culture plate, with an inoculation volume of 100 μl per well.

[0068] 2. Culture A549 cells: at 37°C, 5% CO 2 Under culture conditions, culture for 1 day.

[0069] 3. Add 18β-glycyrrhetinic acid: discard the DMEM medium in each well, add 100 μl to each well and dilute to corresponding concentration (10 μM, 25 μM, 50 μM, 100 μM, 200 μM) of 18β-glycyrrhetinic acid (18β-GRA), and 100 μl of DMEM medium containing 5% fetal bovine serum without drug was added to the control well.

[0070] 4. Coloring: After 24 hours of culture, add 10 μl of MTT solution to each well, and incubate at 37°C, 5% CO 2 Continue to incubate under the culture conditions for 4 hours, then terminate the culture, dis...

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Abstract

The invention belongs to the technical field of medicine application, and discloses application of carbenoxolone in the preparation of an anti-Zika virus medicine. An in-vitro inhibition experiment ofa Zika virus (ZIVK) proves that carbenoxolone has a good inhibition effect on the ZIVK; by constructing a ZIKV infected animal model, it is discovered that ZIKV causes animal cephalotaxis and brain development of animals infected with ZIKV is obviously delayed, and through administration of carbenoxolone to the animals, the phenomenon of brain development retardation of the ZIKV infected animalscan be significantly improved. Essentially, it is proved that the medicine has wide application prospects in treatment of Zika virus infection diseases. Meanwhile, the carbenoxolone comes from liquorice, and thus is rich in source and convenient to acquire; according to the present invention, the good post-selection drug can be provided for clinical treatment of the diseases caused by the Zika virus, and the good application prospect is provided.

Description

technical field [0001] The present invention relates to the technical field of drug application, more specifically, relates to the application of carbenoxic acid in the preparation of anti-Zika virus drugs. Background technique [0002] Glycyrrhizinic acid (carbenoxolone) is the disodium salt of glycyrrhetinic acid hemisuccinate. Glycyrrhetinic acid is a compound produced by removing 2 molecules of glucuronic acid after hydrolysis of glycyrrhizinic acid and its salt (glycyrrhizin). The chemical constituents of licorice mainly exist in the rhizomes and roots of licorice. The chemical formula of carbenic acid is: C 34 h 48 o 7 Na 2 , Molecular weight: 614.7, carbenoxic acid is a colorless powder, tasteless, soluble in water to form a yellowish liquid. [0003] There are two common medical uses of carbenoxic acid: 1. As a gap junction blocker, it is used to reduce the material conduction between cells, and it is mainly used for experimental research on heart, embryo, tumor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61K31/704A61P31/14
CPCA61K31/56A61K31/704A61P31/14Y02A50/30
Inventor 黄曦刘婷
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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