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A gene related to iron content in pear tree leaves and its application

A technology of iron content and leaves, applied in the field of agricultural cultivation, can solve the problem that the cause of pear tree leaf yellowing cannot be quickly judged, and achieve the effect of avoiding tediousness and improving accuracy

Active Publication Date: 2021-11-23
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a gene related to the iron content of pear leaves and its application to solve the technical problem that the cause of pear leaf yellowing cannot be quickly judged in the prior art

Method used

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  • A gene related to iron content in pear tree leaves and its application
  • A gene related to iron content in pear tree leaves and its application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0037] (1) cDNA extraction

[0038] Leaf RNA was obtained from pear leaves with a polysaccharide and polyphenol plant total RNA extraction kit (DP441-RNA prep pure, Tiangen), and leaf cDNA was synthesized with a common reverse transcription kit (AH311-02, full gold). The cDNA concentration of each leaf sample was checked using a Beckman Coulter DU800 UV-Vis spectrophotometer (Beckman, Pasadena, CA, USA). RNase-Free H 2 O Adjust the cDNA concentration to 2000ng / μL, and store at -80°C for later use.

[0039] (2) PCR reaction

[0040] The total volume of reaction components is 50 μL, containing 25 μL 2×KOD Neo buffer (TOYOBO), 1 μL of the above sample cDNA, 10 μL 2mM dNTPs, 1 μL 0.50 μM LOC-F / R specific primer, 1 μL KOD Neo, add deionized sterile water to the final volume . The C1000 gradient thermal cycler (Bio-Rad Laboratories srl) was used for amplification. The reaction conditions were: initial denaturation at 95°C for 3 minutes; continuous denaturation at 95°C for 30 sec...

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Abstract

The invention discloses a gene related to the iron content of pear tree leaves and its application, and relates to the field of agricultural cultivation technology. The invention discovers a gene related to the iron content of pear tree leaves. The nucleotide sequence of the gene is as shown in SEQ ID As shown in NO 1, at the same time, the present invention provides a method for detecting the etiology of pear tree yellowing by using the above sequence, the method specifically includes collecting the cDNA of pear tree, designing primers to amplify the above sequence, and using different methods for the amplified product. Two different amplified products were amplified by the primer pair, and vectors were prepared. Finally, two kinds of suspensions were prepared and injected into the yellowed leaves and normal leaves of Huanghua pear tree respectively. According to the results after injection, it can be quickly judged Whether the pear tree yellowing is caused by iron deficiency, the method provided by the invention is simple and effective, and provides an effective judgment basis for treating the pear tree yellowing.

Description

technical field [0001] The invention relates to the technical field of agricultural cultivation, in particular to a gene related to pear tree yellowing disease and a method for judging the cause of pear tree leaf yellowing disease. Background technique [0002] my country is a big pear planting country in the world, and its cultivation area and output rank first in the world. The area of ​​the old course of the Yellow River is one of the important pear cultivation areas in my country. Most of them are alkaline soils, and the occurrence of yellow leaf disease caused by iron deficiency in pear orchards is relatively common. In recent years, in sand pear orchards with acidic soil in the south, yellow leaf disease caused by iron deficiency has also occurred, becoming one of the important physiological diseases in industrial development. [0003] In the past, the diagnosis of pear tree yellow leaf disease mainly used the determination of Fe content in soil and Fe content in leav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 贾兵郭国凌魏鹏飞朱立武叶振风衡伟刘莉
Owner ANHUI AGRICULTURAL UNIVERSITY