Nucleic acid testing kit for freeze-dried novel coronaviruses, influenza viruses and hepatitis viruses and detection method

Abstract

The invention relates to the technical field of nucleic acid testing, and discloses a nucleic acid testing kit for freeze-dried novel coronaviruses, influenza viruses and hepatitis viruses. The nucleic acid testing kit comprises a rectangular paper box package, wherein sponge with three grooves is arranged in the rectangular paper box package, and a penicillin bottle filled with a full-component novel coronavirus freeze-drying reaction system, a plastic centrifuge tube filled with a positive control substance and a plastic centrifuge tube filled with a negative control substance are respectively inserted into three grooves of the sponge. Compared with an enzyme-linked immunosorbent assay, the nucleic acid testing kit utilizing a freeze-drying RT-PCR nucleic acid testing technique is stronger in specificity and higher in sensitivity; compared with a colloidal gold immunochromatography, the nucleic acid testing kit is higher in accuracy; compared with a conventional PCR method, the consumed time is shorter; and compared with the prior art, the biological activity of a thermosensitive biological product is protected by the full-component novel coronavirus freeze-drying reaction systemof the nucleic acid testing kit, and the moisture content of the freeze-dried product is extremely low, so that the stability of the product is improved, the pollution possibility is reduced, the preservation of the product is promoted, the product is convenient to transport, and the quality guarantee period of the product is prolonged.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a freeze-dried novel coronavirus, influenza virus, pneumonia virus nucleic acid detection kit and detection method. Background technique [0002] In December 2019, a new type of pneumonia appeared. The main transmission routes are respiratory droplet transmission and contact transmission, aerosol and fecal-oral transmission routes. On January 12, 2020, the new type of coronavirus was tentatively named " 2019-nCoV", which mainly causes fever, dry cough, and fatigue. As of 8:50 on June 29, 2020, Beijing time, a total of 10,174,300 cases have been diagnosed worldwide; there is no clear treatment for the disease, and nucleic acid The detection method is the most effective detection method, which is an important reference for clinical diagnosis. [0003] At present, ELISA (enzyme-linked immunoassay), colloidal gold immunochromatography, conventional PCR, and RT-PCR are...

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a freeze-dried novel coronavirus, influenza virus, pneumonia virus nucleic acid detection kit and detection method, which solves the problems of poor specificity, poor sensitivity, and accuracy in the prior art. low and time-consuming issues

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