Application of siRNA sequences and target thereof in improving PEDV titer
A technology of sequence and toxicity, which is applied in the application field of siRNA sequence and its target in improving the toxicity of PEDV, which can solve the problems of complicated downstream signaling pathways and complex LncRNA structure.
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[0057] Example 1:
[0058] 1. Expression of LNC_002015 in Vero-E6 cells at different infection time periods
[0059] Taking the PEDV-infected Vero-E6 cells with MOI=0.01 as a model, the cells with the highest intracellular virus titer were selected as the experimental group, and the uninfected cells at the same time period were used as the blank control, and the second-generation whole transcriptome high-throughput was performed After sequencing, the sequencing data was analyzed and processed, and Vero-E6 cells were screened to significantly up-regulate the expression of LNC_002015, and the expression in different time periods was detected (the primers for detecting LNC_002015 are shown in Table 1).
[0060] Table 1. Detection primers and probes
[0061]
[0062] The experimental group was the Vero-E6 cells inoculated with CV777 with MOI=0.01, and the blank control group was the control group without CV777 inoculated Vero-E6 cells. They were discarded at 12, 24, 36, 48, 60, 72h after ...
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