A microfluidic chip, system and application for juvenile zebrafish

A microfluidic chip and microfluidic technology, applied in applications, laboratory containers, fish farming, etc., can solve problems such as complex structures and external systems, and difficulty in simultaneously completing multiple physiological information records

Active Publication Date: 2022-04-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the structures and external systems of most microfluidic chips on the market are not only relatively complex, but also difficult to record multiple physiological information at the same time (such as animal behavior information such as tail swings, fin strokes, and mouth opening and closing, heartbeat information, etc. blood flow information, EEG information, etc.)

Method used

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  • A microfluidic chip, system and application for juvenile zebrafish
  • A microfluidic chip, system and application for juvenile zebrafish
  • A microfluidic chip, system and application for juvenile zebrafish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1 A microfluidic chip for juvenile zebrafish

[0079] The microfluidic chip for juvenile zebrafish in this embodiment includes a microfluidic substrate and a microfluidic negative placed under the microfluidic substrate;

[0080] The microfluidic substrate is provided with a reaction channel with a 500 μm depression upward from the bottom surface;

[0081] The periphery of the microfluidic substrate is provided with a liquid inlet channel and a liquid outlet channel communicated with the reaction channel;

[0082] The reaction channel is sequentially provided with a fixed cavity for fixing the head of zebrafish larvae, a restricted passage through which only the tail of zebrafish larvae can pass, and an active cavity for the activity of the tail of zebrafish larvae along the direction of fluid flow;

[0083] The microfluidic substrate is made of polydimethylsiloxane;

[0084] The microfluidic bottom sheet is made of glass.

[0085] The liquid inlet channel is...

Embodiment 2

[0098] Example 2 A microfluidic chip for juvenile zebrafish

[0099] The microfluidic chip for juvenile zebrafish in this embodiment includes a microfluidic substrate and a microfluidic negative placed under the microfluidic substrate;

[0100] The microfluidic substrate is provided with a reaction channel with a 600 μm depression upward from the bottom surface;

[0101] The periphery of the microfluidic substrate is provided with a liquid inlet channel and a liquid outlet channel communicated with the reaction channel;

[0102] The reaction channel is sequentially provided with a fixed cavity for fixing the head of zebrafish larvae, a restricted passage through which only the tail of zebrafish larvae can pass, and an active cavity for the activity of the tail of zebrafish larvae along the direction of fluid flow;

[0103] The microfluidic substrate is made of polydimethylsiloxane;

[0104] The microfluidic bottom sheet is made of glass.

[0105] The liquid inlet channel is...

Embodiment 3

[0115] Example 3 A microfluidic chip for juvenile zebrafish

[0116] The microfluidic chip for juvenile zebrafish in this embodiment includes a microfluidic substrate and a microfluidic negative placed under the microfluidic substrate;

[0117] The microfluidic substrate is provided with a reaction channel with a 600 μm depression upward from the bottom surface;

[0118] The periphery of the microfluidic substrate is provided with a liquid inlet channel and a liquid outlet channel communicated with the reaction channel;

[0119] The reaction channel is sequentially provided with a fixed cavity for fixing the head of zebrafish larvae, a restricted passage through which only the tail of zebrafish larvae can pass, and an active cavity for the activity of the tail of zebrafish larvae along the direction of fluid flow;

[0120] The microfluidic substrate is made of polydimethylsiloxane;

[0121] The microfluidic bottom sheet is made of glass.

[0122] The liquid inlet channel is...

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Abstract

The invention discloses a microfluidic chip for zebrafish larvae, a system and an application thereof. The microfluidic chip in the present invention is composed of a plurality of specially designed microstructure arrays, and each microstructure has a liquid inlet and outlet channel and a zebrafish juvenile reaction channel connected. The present invention aims to carry out precise micro-manipulation, partial fixation and collection of physiological information on zebrafish seedlings through the microfluidic chip, and at the same time realize the recording of various physiological information, so as to realize in-depth research on animal systems and comprehensive evaluation of drugs.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip for juvenile zebrafish, a system and an application thereof. Background technique [0002] Traditional drug screening methods are mostly based on in vitro detection of cell or molecular targets, but due to the limited number of cells or molecules and the difficulty of fully simulating the microenvironment of a specific system (such as the central nervous system), the efficiency of drug screening is extremely low. At present, the emergence of in vivo drug screening methods based on whole animals, such as zebrafish eggs, nematodes and fruit flies, has brought a new direction for improving the success rate of drug screening. [0003] Zebrafish embryos (fish eggs) have been widely used in the study of living systems based on single embryos due to their high transparency, strong permeability, small size, and easy handling. However, zebrafish eggs also d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00A61D3/00A01K61/10
CPCA01K61/10B01L3/502707Y02A40/81
Inventor 林旭东唐明卉
Owner SUN YAT SEN UNIV
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