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Preparation method of eimeria tenella recombinant polypeptide vaccine VKVQ and application method of eimeria tenella recombinant polypeptide vaccine VKVQ in resisting chicken coccidiosis

A technology of Eimeria and recombinant polypeptides, applied in the fields of botany equipment and methods, biochemical equipment and methods, applications, etc.

Active Publication Date: 2020-10-30
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
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Problems solved by technology

However, to date, there are no other studies on the immunoprotective properties of this protein against Eimeria

Method used

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  • Preparation method of eimeria tenella recombinant polypeptide vaccine VKVQ and application method of eimeria tenella recombinant polypeptide vaccine VKVQ in resisting chicken coccidiosis
  • Preparation method of eimeria tenella recombinant polypeptide vaccine VKVQ and application method of eimeria tenella recombinant polypeptide vaccine VKVQ in resisting chicken coccidiosis
  • Preparation method of eimeria tenella recombinant polypeptide vaccine VKVQ and application method of eimeria tenella recombinant polypeptide vaccine VKVQ in resisting chicken coccidiosis

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Embodiment Construction

[0018] In order to make the technical means realized by the invention, creation features, goals and effects easy to understand, the present invention will be further elaborated below in conjunction with specific illustrations. The molecular biology experiment techniques adopted in the following examples include PCR amplification, plasmid extraction, Plasmid transformation, DNA fragment ligation, restriction enzyme digestion, gel electrophoresis, etc. all adopt routine methods. For details, please refer to "Molecular Cloning Experiment Guide" (Third Edition) (Sambrook J, Russell DW, Janssen K, Argentine J. Huang Peitang et al. translation , 2002, Beijing: Science Press). And "Modern Molecular Biology Experimental Technology" (Edited by Lu Shengdong, 2nd edition, Beijing, China Peking Union Medical College Press, 1999.).

[0019] A preparation method of Eimeria tenella recombinant polypeptide vaccine VKVQ, comprising:

[0020] 1. Cloning of Et-VKVQ coding gene:

[0021] 1.1 Pr...

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Abstract

The invention discloses a preparation method of an eimeria tenella recombinant polypeptide vaccine VKVQ. The preparation method comprises the following steps: 1, cloning a VKVQ encoding gene, designing an upstream primer shown in the formula SEQ ID NO: 3 and a downstream primer shown in the formula SEQ ID NO: 4 according to a chicken coccidiosis gene sequence, carrying out PCR amplification and electrophoresis to obtain a segment of about 1200bp, carrying out ligation transformation on the product to obtain VKVQ of which the sequence overall length is 1245bp and of which the sequence is shownin the formula of SEQ ID NO: 1, and carrying out subsequent related steps. The vaccine prepared by the method can effectively control chicken bodies to be infected with eimeria tenella by immunizing eimeria tenella recombinant polypeptide vaccine VKVQ eukaryotic expression plasmids and prokaryotic expression proteins, so that the use amount of anti-coccidiosis drugs in chicken farms is greatly reduced, and chicken coccidiosis is effectively controlled.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, and more specifically relates to the preparation of a recombinant Eimeria tenella polypeptide vaccine VKVQ and its application method in chicken coccidiosis resistance. Background technique [0002] Eimeria tenella (E.tenella) is a kind of obligate intracellular parasitic apicomplexan protozoa, which can cause serious damage to chicken coccidiosis in intensive chicken industry. In the absence of preventive measures or failure of prevention (such as drug ineffective due to drug resistance problems), the incidence rate of chickens can reach 30%-100%, and the lethal rate can be as high as 80%. The global economic loss caused by chicken coccidiosis exceeds more than 3 billion US dollars every year. At present, the prevention and control of coccidiosis in chickens is still mainly carried out by the technical methods of "adding various anticoccidial drugs to the feed for drug pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/012A61K39/39A61P33/02C07K14/455C12N15/30C12N15/85
CPCA61K39/012A61K39/39A61P33/02C07K14/455C12N15/85A61K2039/552A61K2039/55566Y02A50/30
Inventor 戚南山孙铭飞廖申权吕敏娜吴彩艳李娟林栩慧胡俊菁蔡海明肖文婉于林增张健騑
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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