On-site detection kit and detection method for vibrio parahaemolyticus in water body

Abstract

The invention relates to an on-site detection kit and a detection method for vibrio parahaemolyticus in a water body, and belongs to the field of water quality detection. The kit comprises a brominated N-dodecyl isoquinoline reagent, a vibrio parahaemolyticus virulent phage suspension and vibrio parahaemolyticus bacterial suspensions with different concentration gradients. The method for detectingthe vibrio parahaemolyticus in the water body by utilizing the kit comprises the steps of filling a water sample to be detected into a detection tube, and adding the special virulent bacteriophage, so that the vibrio parahaemolyticus in the water sample can be specifically and quickly split to generate various small molecules and ions, and the [sigma] value is increased. As the increment of [sigma] is in direct proportion to the amount of the vibrio parahaemolyticus in the water body contained in the detection tube, the quantitative information of the vibrio parahaemolyticus in the water bodysample to be detected can be calculated by measuring [delta]sigma. According to the method, the amount of the vibrio parahaemolyticus in the complex water body containing various microorganisms can be rapidly determined on a culture site, and high-timeliness and accurate support data is provided for fishery production management.

Description

Technical field: [0001] The invention belongs to the field of water quality detection, and in particular relates to an on-site detection kit and detection method for Vibrio parahaemolyticus in water bodies. Background technique: [0002] Vibrio parahaemolyticus (Vibrio parahaemolyticus) is a Gram-negative bacterium, halophilic, and widely exists in fishery environments and aquatic organisms. The common methods for detecting Vibrio parahaemolyticus in water are mainly based on enrichment culture-isolation culture-biochemical The national standard (GB4789.7-2013) method of the reaction. Although this method is the gold standard, the operation is complicated, labor-intensive, and the cycle is long, and the accuracy depends heavily on the professional level of the operator, so it cannot achieve on-site, rapid and accurate determination. In recent years, molecular biology methods based on gene detection (Huang Mengshi, Yang Qianqian, Zhang Yan, Gu Yangguang, Zhao Jun, Zhang Xuzh...

AI Technical Summary

Problems solved by technology

However, the new method is limited by expensive instruments and professional analysis sites, and it is still not possible to achieve on-site, rapid and accurate determination of Vibrio parahaemolyticus

Therefore, it is not yet possible to provide effective early warning data for aquaculture and other production activities

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