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Method for preparing universal immune cells and application of universal immune cells

A general-purpose technology of immune cells, applied in the field of biomedicine, can solve the problems of not seeing iPSCs, etc., and achieve the effect of improving the immune function of the body, easy to operate, and simple methods

Pending Publication Date: 2020-11-03
SHANGHAI XINGHUA BIOMEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is no report of iPSC-derived CTL (iPSC-CTL)

Method used

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  • Method for preparing universal immune cells and application of universal immune cells
  • Method for preparing universal immune cells and application of universal immune cells
  • Method for preparing universal immune cells and application of universal immune cells

Examples

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preparation example Construction

[0050] The first aspect of the present invention is to provide a convenient method for preparing general-purpose immune cells. Allogeneic mononuclear cells are co-cultured with cell mitogens, cytokines and immune adjuvants to obtain a large number of general-purpose immune cells. The method specifically includes the following steps:

[0051] Step 1, allogeneic immune cells (such as mononuclear cells) are co-cultured with cell mitogens, cytokines and immune adjuvants in a liquid cell culture medium in a culture vessel to obtain a general-purpose immunogenic immune cell cultures;

[0052] Step 2, isolating immunocompetent cell populations from the culture obtained in step 1;

[0053] Among them, the cytokines are derived from any one or several of lymphokines, monokines, cytokines that activate inflammation, and cytokines that stimulate hematopoiesis produced by lymphocytes, monocytes and other cells.

[0054] In a preferred embodiment of the present invention, the above-menti...

Embodiment 1

[0085] This example provides a simple method for preparing general-purpose immune cells, including the following steps:

[0086] Allogeneic mononuclear cells derived from normal human peripheral blood are co-cultured with cell mitogens, cytokines and immune adjuvants in a liquid medium, and the amount of cell mitogen concanavalin in the medium is 100,000 units / L The amount of cytokine interleukin-2 in the medium is 500,000 units / L; the amount of immune adjuvant (5% Tween-80) is 0.5mL / L.

[0087] Allogeneic mononuclear cells were co-cultured with cell mitogens, cytokines and immune adjuvants for 30 days to obtain immunocompetent immune cell cultures, mainly cytotoxic T lymphocytes.

Embodiment 2

[0089] This example provides a simple method for preparing general-purpose immune cells, including the following steps:

[0090] Mononuclear cells derived from allogeneic peripheral blood are co-cultured with cell mitogens, cytokines and immune adjuvants in a liquid medium, and the medium includes: cell mitogen phytohemagglutinin 0.5mg / L; The cytokine interferon was 5 million units / L; the dosage of immune adjuvant (5% Tween-80) was 0.1 mL / L.

[0091] Allogeneic mononuclear cells were co-cultured with cell mitogens, cytokines and immune adjuvants for 30 days to obtain immunocompetent immune cell cultures, mainly cytotoxic T lymphocytes.

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Abstract

The invention provides a simple and convenient method for preparing universal immune cells and an application of the universal immune cells. According to the method, allogeneic immune cells, specificcell mitogens, cell factors and immunologic adjuvants are placed in a liquid cell culture medium to be co-cultured in a culture container, so that universal immune cell culture with high immunocompetence is obtained. The method is simple, convenient and easy to operate, the allogeneic immune cells are adopted, the quality of immune cell preparations can be improved, the cost of cell therapy is reduced, and the safety of the cell therapy is improved. In addition, a large number of clinical long-term practical applications prove that the universal immune cells are safe, reliable, durable and effective, and have no rejection reaction. Therefore, the universal immune cells can be used as maternal cells of other types of universal immune cell preparations, such as CAR-T and TCR-T, and can be used in the fields of adoptive cellular immunotherapy and the like. According to the invention, the problems of high cost, poor safety, low efficiency and the like of existing cellular immunotherapy aresolved, and a new way is provided for application and popularization of the cellular immunotherapy.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing universal immune cells and its application. Background technique [0002] In recent decades, great progress has been made in the field of cancer treatment. In particular, cell-based therapies targeting tumor-associated antigens (TAAs) have developed rapidly. T cells were engineered to have the ability to attack tumor cells by generating a CAR construct consisting of a gene encoding a scFv, a co-stimulatory domain (CD28 or TNFRSF9), and a CD247 signaling domain for T cell proliferation and activation. In principle, CAR-T cells are activated by recognition of TAA by scFv on the surface of T cells, and the signaling domains within the cells linked by the scFv are subsequently activated to induce downstream signaling involved in T cell proliferation, activation, and cytokine production way. [0003] Currently, the potent function of CAR-T cell therapy has been dem...

Claims

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Application Information

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IPC IPC(8): C12N5/0783C12N5/0786C12N5/0784A61K39/00A61K35/17A61K35/15A61P35/00A61P31/18A61P1/16A61P31/14A61P31/20A61P31/12A61P39/06
CPCC12N5/0638C12N5/0646C12N5/0645C12N5/0639A61K39/0011A61K39/0005A61K35/17A61K35/15A61K9/0019A61P35/00A61P31/18A61P1/16A61P31/14A61P31/20A61P31/12A61P39/06C12N2501/2302C12N2501/2306C12N2501/2315C12N2501/24C12N2501/90C12N2501/998A61K2039/54
Inventor 刘华
Owner SHANGHAI XINGHUA BIOMEDICAL TECH
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