Method for preparing pro-xylane from biological enzyme by one-pot method

A technology of biological enzyme and boson, which is applied in the field of enzyme engineering to achieve the effect of safe environment, simple, reliable and direct method, and beneficial to industrial production.

Pending Publication Date: 2020-11-03
江苏瑞蓓丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the biosynthesis method of domestic production of Boseine has not been reported,

Method used

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  • Method for preparing pro-xylane from biological enzyme by one-pot method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A one-pot method for preparing Boseine using biological enzymes, the method includes the following steps:

[0018] (1) Preparation of substrate solution: Dissolve 100g of xylose in 1L of pH 6.0 0.1mM phosphate buffer to make the concentration of xylose 100g / L, and then add isopropanol to make the concentration of isopropanol 50g / L, and stir well.

[0019] (2) Enzyme-catalyzed preparation of Bosein: Add 10g / L biological enzyme preparation to the substrate solution. In biological enzyme preparations, isopropanol dehydrogenase, Bosein synthase, and carbonyl reductase, the mass composition ratio of these three enzyme powders is 1:1:1. At the same time, the coenzyme nicotinamide adenine dinucleotide was added to make its concentration 10mM. The conditions of the catalytic reaction were: the temperature was 30°C, the stirring speed was 50 rpm, and the reaction time was 24 h.

[0020] (3) Ultrafiltration to remove enzymes: the step (2) enzyme-catalyzed reaction solution is subjec...

Embodiment 2

[0025] A one-pot method for preparing Boseine using biological enzymes, the method includes the following steps:

[0026] (1) Preparation of substrate solution: Dissolve 200g of xylose in 1L of pH7.0 0.1mM phosphate buffer to make the concentration of xylose 200g / L, and then add isopropanol to make the concentration of isopropanol 100g / L, and stir well.

[0027] (2) Enzyme-catalyzed preparation of Bosein: Add 20g / L biological enzyme preparation to the substrate solution. In biological enzyme preparations, isopropanol dehydrogenase, Bosein synthase, and carbonyl reductase, the mass composition ratio of these three enzyme powders is 1:2:1. At the same time, the coenzyme nicotinamide adenine dinucleotide was added to make its concentration 20mM. The conditions of the catalytic reaction were as follows: the temperature was 35°C, the stirring speed was 100 rpm, and the reaction time was 36 h.

[0028] (3) Ultrafiltration to remove enzymes: the step (2) enzyme catalyzed reaction soluti...

Embodiment 3

[0033] A one-pot method for preparing Boseine using biological enzymes, the method includes the following steps:

[0034] (1) Preparation of substrate solution: Dissolve 100g of xylose in 1L of pH7.0 0.2mM phosphate buffer to make the concentration of xylose 100g / L, and then add isopropanol to make the concentration of isopropanol 100g / L, and stir well.

[0035] (2) Enzyme-catalyzed preparation of Bosein: Add 20g / L of biological enzyme preparation to the substrate solution. In biological enzyme preparations, isopropanol dehydrogenase, Bosein synthase, and carbonyl reductase, the mass composition ratio of these three enzyme powders is 1:0.5:1. At the same time, the coenzyme nicotinamide adenine dinucleotide was added to make its concentration 20mM. The catalytic reaction conditions are: temperature is 35°C, stirring speed is 50 rpm, and reaction time is 36 h.

[0036] (3) Ultrafiltration to remove enzymes: the step (2) enzyme-catalyzed reaction solution is subjected to ultrafiltra...

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Abstract

The invention discloses a method for preparing pro-xylane from a biological enzyme by a one-pot method. The method is characterized by comprising the following steps of taking xylose and isopropanol as substrates, generating the pro-xylane under the catalytic action of isopropanol dehydrogenase, pro-xylane synzyme, carbonyl reductase and coenzyme nicotinamide adenine dinucleotide. The method is simple in process, free of chemical reagents, low in cost, high in yield and suitable for industrial production.

Description

Technical field [0001] The invention relates to the technical field of enzyme engineering, in particular to a method for preparing Boseine by using a one-pot method of biological enzymes. Background technique [0002] Bosein, the chemical name is hydroxypropyl tetrahydropyrantriol, and the CAS number is 439685-79-7. Bosein is an active substance commonly used in cosmetics. It is made by L'Oreal extracted from Western European Beech. Lancome was the first to use Bosein in skin care products. Bosein can promote the synthesis of mucopolysaccharides in the skin, promote the production of hyaluronic acid and collagen, improve the adhesion between the dermis and the epidermis, promote the regeneration of damaged tissues, help maintain the elasticity of the dermis, and prevent skin aging. At present, the biosynthetic method of producing bosein in China has not been reported, and only a small amount of literature discloses the method of chemical synthesis of bosein. [0003] Patent docu...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12N9/04
CPCC12P17/06C12N9/0006C12Y101/0108C12Y101/01184
Inventor 李赟高蔡衡其他发明人请求不公开姓名
Owner 江苏瑞蓓丽生物科技有限公司
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