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Copeptin antibody preparation and detection establishing method

A peptide antigen, monoclonal antibody technology, applied in the field of peptide chemistry and immunology, can solve problems such as instability

Pending Publication Date: 2020-11-06
JILIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is challenging to detect the level of AVP in the blood circulation, because AVP is an unstable short peptide with a half-life of less than 20 minutes, and most of AVP is bound to platelets, so it is necessary to find a surrogate indicator of AVP

Method used

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  • Copeptin antibody preparation and detection establishing method
  • Copeptin antibody preparation and detection establishing method
  • Copeptin antibody preparation and detection establishing method

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Embodiment Construction

[0024] In order to establish a detection method for CPP, the present invention provides a human CPP antigen epitope peptide antigen for producing CPP monoclonal antibody or polyclonal antibody, using the antibody to establish a detection method for CPP and a CPP in vitro diagnostic kit.

[0025] Specifically, the invention discloses a preparation process of a CPP antigen epitope peptide antigen, the steps are as follows:

[0026] Synthetic CPP epitope peptide (SEQ ID NO.1), the amino acid sequence is 126-164:

[0027] CASDRSNATQLDGPAGALLLLRLVQLAGAPEPFEPAQPDAY

[0028] Synthesized CPP-N epitope peptide (SEQ ID NO.2), the amino acid sequence is 131-140:

[0029] CKIEISNATQLDGPA

[0030] Synthesized CPP-M peptide (SEQ ID NO.3), the amino acid sequence is 131-140:

[0031] FHYKSNATQLDGPAGALL

[0032] The N-terminal of the CPP-N antigenic epitope peptide of the present invention adds 5 additional amino acids, and the outermost end is Cys amino acid, and the N-terminal of the CP...

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Abstract

The invention discloses a copeptin antibody preparation and detection establishing method, which belongs to the field of peptide chemistry and immunology, and aims to provide a human CPP antigen epitope peptide antigen used for generating a CPP monoclonal antibody or a polyclonal antibody, and establishing a CPP detection method and a CPP in-vitro diagnostic kit by using the antibody. According tothe CPP antigen epitope peptide, the CPP-N antigen epitope peptide is synthesized, and the CPP-M peptide is synthesized. The specific CPP antigen is prepared; a CPP monoclonal antibody or a polyclonal antibody is generated and used for establishing a CPP detection method and a CPP in-vitro diagnostic kit, and one is an immunochromatographic test strip for rapidly and quantitatively detecting CPPconcentration, is used for detecting the CPP level in blood or body fluid and is used for judging the occurrence of AMI, stroke and other diseases.

Description

technical field [0001] The present invention is in the field of peptide chemistry and immunology. Background technique [0002] The Arginine Vasopressin (AVP) gene is located on chromosome 20 and encodes a 164-amino acid peptide called pro-arginine vasopressin, which is produced by neurons of the hypothalamus-neurohypophysis system. The structure of pro-arginine vasopressin includes signal peptide, AVP, neurophysics IIN (Neuropathysin II) and copeptin (CPP). [0003] AVP contains a short peptide of 9 amino acids, also known as antidiuretic hormone or vasopressin, AVP can interact with V1a, V2, V1b receptors to exert its biological effects: V1a receptors have a strong contraction of small arteries ; Exciting V2 receptors can produce anti-diuretic effect, promote kidney water retention, maintain osmotic pressure and cardiovascular homeostasis in the body; V1b receptors exist in the pituitary gland and islet cells, and have endocrine mediation activity. AVP is a peptide hormo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/543G01N33/533
CPCG01N33/6893G01N33/577G01N33/558G01N33/54313G01N33/533
Inventor 王珺楠于宏波李宝民宋阳关恒刘斌艾清张蕾黄景林张金玲赵青刘戈仇淑园史永丰王贺朱道林
Owner JILIN UNIV
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