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Sclera tissue preservation method

A preservation method and sclera technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of limiting the clinical application of active tissues, complex procedures, and difficult operations, and achieve easy implementation, simple production process, and structural The effect of less damage

Inactive Publication Date: 2020-11-10
XIAMEN EYE CENTER OF XIAMEN UNIVERSITY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] During the preservation period of traditional sclera materials, they are mainly preserved according to the preservation method of corneal materials. The specific method is: long-term freezing and dehydration after removing ocular tissues such as conjunctiva, extraocular muscles, iris, ciliary body, choroid and optic nerve. Complicated, difficult to operate, and to a certain extent limit the clinical application of living tissues

Method used

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Embodiment Construction

[0013] In order to further explain the technical scheme of the present invention, the present invention will be described in detail below through specific embodiments.

[0014] A method for preserving scleral tissue, which is to repeatedly rinse the virus-free sclera and conjunctival tissues after disinfection and air exposure preservation, the air exposure culture is to cover the conjunctiva on the scleral tissue, and then place it in a water-permeable On the film, and under the film is a preservation solution or cell culture medium that can provide tissue nutrients. The scleral tissue preservation method of the present invention can make the preserved conjunctiva and scleral tissue in an artificial wet room environment, which is similar to the surface survival environment of a normal physiological eyeball in the human body, and the scleral cell structure is rarely damaged.

[0015] Said disinfection is to immerse the fresh sclera and conjunctiva with phosphate buffer containing a...

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PUM

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Abstract

The invention discloses a sclera tissue preservation method, which comprises the following steps: disinfecting repeatedly rinsed virus-free fresh sclera and conjunctival tissue, and then carrying outair exposure culture, wherein the air exposure culture comprises: covering sclera tissues with conjunctiva, and then placing the sclera tissues on a water-permeable film, wherein a preserving fluid ora cell culture medium capable of providing tissue nutrient components is arranged below the film. By means of the sclera tissue preservation method, conjunctiva and sclera tissue can be preserved inan artificial wet room environment, the environment is similar to the surface survival environment of normal physiological eyeballs in a human body, sclera cell structure damage is little, and structure preservation is complete. Compared with an existing sclera tissue preservation method, the sclera tissue preservation method of the invention is simple and reliable in manufacturing process, easy to implement, relatively low in cost, easy to popularize and convenient to transport, and can provide important technical support for tissue engineering conjunctiva and sclera industrialization.

Description

Technical field [0001] The invention relates to the technical field of ophthalmology medical treatment, in particular to a method for preserving scleral tissue. Background technique [0002] During the preservation of traditional scleral materials, they are mainly preserved according to the preservation method of corneal materials. The specific method is: removing the conjunctiva, extraocular muscles, iris, ciliary body, choroid and optic nerve and other eye tissues after long-term freezing and dehydration. The process of this preservation method It is complicated, difficult to operate, and limits the clinical application of active tissues to a certain extent. Summary of the invention [0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a method for preserving scleral tissue that is simple, reliable and easy to implement. [0004] In order to achieve the above objective, the solution of the present invention is: [0005] A method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0205A01N1/0215A01N1/0231
Inventor 董诺秦文娟吴护平陈新宇周敏姜焕荣朱燕楠
Owner XIAMEN EYE CENTER OF XIAMEN UNIVERSITY CO LTD