A kind of Pichia pastoris low temperature medium

A culture medium and low-temperature technology, applied in the direction of microorganism-based methods, fungi, microorganisms, etc., to achieve the effect of expanding application conditions, reducing the difficulty of downstream purification treatment, and improving purity
CN111909859BActive Publication Date: 2022-07-19BEIJING SL PHARMA +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING SL PHARMA
Publication Date
2022-07-19

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Abstract

The invention discloses a low-temperature type medium of Pichia pastoris, which is helpful for realizing the expression of Pichia pastoris while increasing the expression amount of a unit cell under the condition of low temperature (18-22° C.). High-density fermentation; it can also inhibit various proteases in the fermentation broth, which is conducive to maintaining the stability of the product structure and reducing the generation of product-related impurities; when applied to industrial preparation, it can significantly improve the expression of exogenous proteins yield and reduce the difficulty of downstream purification. The preparation cost and processing difficulty of genetically engineered medicines, enzyme preparations and other products using Pichia pastoris as a host are reduced.
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Description

technical field

[0001] The invention belongs to the field of biological fermentation, and relates to a medium suitable for culturing Pichia pastoris and expressing exogenous proteins under low temperature (18-22°C) conditions. The composition, pH and culture conditions of the medium are included.

[0002] technical background

[0003] In recent years, Pichia pastoris has been widely used in biopharmaceutical, enzyme preparation and other industries, and is considered to be one of the most promising heterologous protein production systems. Researchers generally refer to the "Pichia Fermentation Manual" launched by Invitrogen for fermentation and induction of such engineered bacteria. BSM+PTM is given in this manual 1 It is recommended that the culture temperature be controlled at 30°C. Under such high temperature culture conditions, Pichia pastoris can often reach a high density of 500 g / L wet cells, which is conducive to the increase of the total amount of exogenous protei...

Claims

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