Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Genetic transformation method of haematococcus pluvialis

A genetic transformation method and technology of Haematococcus pluvialis, applied in the field of genetic transformation of Haematococcus pluvialis, can solve the problems of low positive transformation rate, cumbersome operation, low success rate, etc., achieve broad market prospects, avoid cell stress The effect of response and improving the positive rate

Active Publication Date: 2020-11-10
SHENZHEN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects of the genetic transformation method of Haematococcus pluvialis in the prior art, such as cumbersome operation, high cost, low positive transformation rate and low success rate, thereby providing a kind of Haematococcus pluvialis Methods of genetic transformation of algae

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetic transformation method of haematococcus pluvialis
  • Genetic transformation method of haematococcus pluvialis
  • Genetic transformation method of haematococcus pluvialis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The present embodiment provides a method for genetic transformation of Haematococcus pluvialis, comprising the following steps:

[0062] 1 Linearization of plasmids for transformation

[0063] The plasmid used in this example is pH124. Use the plasmid extraction kit Plasmid Mini Kit II (Omega (Omega Bio-tek), the United States) to extract the pH124 plasmid from Escherichia coli DH5α, linearize the plasmid with the restriction endonuclease Not I, and digest the plasmid with Not I The system is 30 μL, specifically including: 20 μL DNA solution, 2 μL Not I fast cutting enzyme (New England (NEB), USA), 3 μL Cutsmart Buffer (New England (NEB), USA), 5 μL water. The enzyme digestion temperature was 37° C. and the enzyme digestion time was 2 hours to obtain a linearized pH124 plasmid having the DNA sequence shown in SEQ ID NO: 1, and the concentration of the linearized plasmid was about 1.0 μg / μL.

[0064] 2 Genetic transformation of Haematococcus pluvialis

[0065] (1) Col...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
strengthaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a genetic transformation method of haematococcus pluvialis. The method comprises the following step of mediating exogenous genes with a polyethylene glycol solution to enter the protoplast of the haematococcus pluvialis. According to the method, the polyethylene glycol solution is adopted to mediate the exogenous genes to be transferred into the protoplast of the haematococcus pluvialis for the first time; due to the fact that the permeability of cell membranes can be improved through polyethylene glycol, linearized plasmids can be transferred into the protoplast of the haematococcus pluvialis more easily; the method does not cause mechanical damage to cells; and the influence on the conversion of the haematococcus pluvialis due to the cell stress reaction caused by damage is avoided. The method solves the problems of complicated operation, high cost, long period and low conversion rate in genegun and agrobacterium tumefaciens mediated genetic transformation. The method can be widely applied to excellent character improvement, gene editing, expression regulation and the like of the haematococcus cells, and has huge commercial advantages and broad market prospects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for genetic transformation of Haematococcus pluvialis. Background technique [0002] As an important economic green algae, Haematococcus pluvialis is the main source of natural astaxanthin. Astaxanthin belongs to carotenoids, which can delay aging, promote antibody production and enhance the body's immunity. It is a natural antioxidant; astaxanthin is also effective in the prevention and treatment of oxidative stress diseases such as human diabetes, gout, hypertension and cancer. Obvious effect, so astaxanthin has great market value, and Haematococcus pluvialis has great research significance. [0003] Most of the Haematococcus pluvialis currently used for commercial production of natural astaxanthin are wild-type varieties obtained by screening. The content of astaxanthin in the cells of this wild-type Haematococcus is very limited (less than 2%). T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12R1/89
CPCC12N15/8206
Inventor 胡章立郭春立王潮岗王会
Owner SHENZHEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com