Application of clemastine fumarate in preparation for resisting methicillin-resistant staphylococcus aureus (MRSA)
A technology of clemastine fumarate and staphylococcus, which is applied in the direction of antibacterial drugs, medical preparations containing active ingredients, active ingredients of heterocyclic compounds, etc., can solve problems such as adverse reactions and side effects, and achieve good inhibitory effect, High safety and good clinical response
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Embodiment 1
[0049] 1. Materials
[0050] 1.1 Drugs and main reagents
[0051] Clemastine fumarate, Meilun Biotechnology Co., Ltd. (Dalian);
[0052] Calcium-adjusted MH Broth Medium (CAMHB), Qingdao High-tech Industrial Park Haibo Biotechnology Co., Ltd. (Qingdao, Shandong);
[0053] MHA agar medium, Qingdao High-tech Industrial Park Haibo Biotechnology Co., Ltd. (Qingdao, Shandong); normal saline, Meilun Biotechnology Co., Ltd. (Dalian);
[0054] 1.2 Experimental strains
[0055] MRSA6 strain isolated and identified by Microbiology Laboratory of Qilu Children's Hospital of Shandong University
[0056] 1.3 Instruments
[0057] Electronic balance (AUY120), SHIMADZU CORPORATION (Japan);
[0058] Bacteria collection ring, Huanyao Pharmaceutical Equipment Sales Co., Ltd. (Beijing, China);
[0059] McFarney Turbidimetric Tube, Kangtai Biotechnology Company (Zhejiang, China);
[0060] Vortex mixer (G-560E), Si company (USA);
[0061] Hot air disinfection box (GRX-9123A), MMM company (Ge...
Embodiment 2
[0076] Example 2 Determination of the dynamic action of clemastine fumarate against MASA
[0077] 1. Materials
[0078] 1.1 Drugs and main reagents
[0079] Clemastine fumarate, Meilun Biotechnology Co., Ltd. (Dalian);
[0080] Calcium-adjusted MH Broth Medium (CAMHB), Qingdao High-tech Industrial Park Haibo Biotechnology Co., Ltd. (Qingdao, Shandong);
[0081] MHA agar medium, Qingdao High-tech Industrial Park Haibo Biotechnology Co., Ltd. (Qingdao, Shandong); normal saline, Meilun Biotechnology Co., Ltd. (Dalian);
[0082] 1.2 Experimental strains
[0083] MASA1 isolated from the microbiology laboratory of Qilu Children's Hospital of Shandong University.
[0084] 1.3 Instruments
[0085] Electronic balance (AUY120), SHIMADZU CORPORATION (Japan);
[0086] Bacteria collection ring, Huanyao Pharmaceutical Equipment Sales Co., Ltd. (Beijing, China);
[0087] McFarney Turbidimetric Tube, Kangtai Biotechnology Company (Zhejiang, China);
[0088] Vortex mixer (G-560E), Si c...
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