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Exopolysaccharide produced by lactobacillus plantarum 589, preparation method, application and composition containing lactobacillus plantarum or exopolysaccharide

A technology of Lactobacillus plantarum and exopolysaccharide, which is applied in the field of biological food and can solve problems that need further research

Active Publication Date: 2020-11-20
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The applicant isolated a new strain of Lactobacillus plantarum (Lactobacillus plantarum) 589 from Sichuan pickle samples in previous research, and conducted research on its application in diarrhea prevention, but whether the strain has the ability to produce exopolysaccharide The ability and whether it has more physiological activities need to be further studied

Method used

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  • Exopolysaccharide produced by lactobacillus plantarum 589, preparation method, application and composition containing lactobacillus plantarum or exopolysaccharide
  • Exopolysaccharide produced by lactobacillus plantarum 589, preparation method, application and composition containing lactobacillus plantarum or exopolysaccharide
  • Exopolysaccharide produced by lactobacillus plantarum 589, preparation method, application and composition containing lactobacillus plantarum or exopolysaccharide

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Embodiment 1

[0044] An exopolysaccharide produced by Lactobacillus plantarum 589, which was isolated by the inventor from a Sichuan pickle sample, identified as Lactobacillus plantarum (Lactobacillus plantarum), named Lactobacillus plantarum 589, and was released on May 25, 2018 It is preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee, and the microorganism preservation number is CGMCC No.15811, which is isolated from a sample of Sichuan pickles in my country by the inventor.

Embodiment 2

[0046] The preparation method of the exopolysaccharide produced by Lactobacillus plantarum 589 comprises the following steps:

[0047] 1) Inoculate the strain of Lactobacillus plantarum 589 in 100 mL of MRS liquid medium at an inoculum amount of 1%, and cultivate it in a constant temperature incubator at 37°C for 14 hours to perform a first-generation activation; Inoculate in 100mL of MRS liquid medium, culture in a constant temperature incubator at 37°C for 14 hours for second-generation activation, and obtain activated bacterial liquid;

[0048] 2) Inoculate the activated bacterial solution of Lactobacillus plantarum 589 in 1L of MRS liquid medium according to the inoculation amount of 1%, and ferment and cultivate it in a constant temperature incubator at 37°C for 24 hours;

[0049] 3) Collect the bacterium liquid of Lactobacillus plantarum 589 after fermentation and culture, boil for 10 minutes to sterilize, then centrifuge to collect the supernatant, add a trichloroacetic...

Embodiment 3

[0052] The preparation method of the exopolysaccharide produced by Lactobacillus plantarum 589 comprises the following steps:

[0053] 1) Cultivate the strain of Lactobacillus plantarum 589 in a constant temperature incubator at 35°C for 20 hours for first-generation activation; then inoculate the bacterial solution obtained from the first-generation activation into 80 mL of MRS liquid medium at a constant temperature of 35°C with an inoculum of 0.8%. Cultivate in the incubator for 20 hours for second-generation activation to obtain activated bacterial liquid;

[0054] 2) Inoculate the activated bacterial liquid of Lactobacillus plantarum 589 in 0.8L MRS liquid medium according to the inoculum size of 0.8%, and ferment and cultivate it in a constant temperature incubator at 35°C for 30h;

[0055] 3) Collect the bacterial liquid of Lactobacillus plantarum 589 after fermentation and culture, boil for 10 minutes to sterilize, then centrifuge to collect the supernatant, and add a ...

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Abstract

The invention relates to the technical field of biological food, and provides exopolysaccharide produced by lactobacillus plantarum 589 in order to further protect the performance of lactobacillus plantarum 589, and the exopolysaccharide has the effect of promoting splenic lymphocyte proliferation. And the exopolysaccharide is obtained by culturing in an MRS liquid culture medium after activating.According to the invention, the performance of the lactobacillus plantarum 589 is deeply expanded, the exopolysaccharide produced by the lactobacillus plantarum 589 and the method for producing the exopolysaccharide at high yield are provided, and the yield of the exopolysaccharide reaches about 580mg / L; the lactobacillus plantarum 589 and derivatives thereof can promote proliferation of spleniclymphocytes; exopolysaccharide produced by the lactobacillus plantarum 589 has a low dosage concentration of only 1 [mu]g / mL for promoting splenic lymphocyte proliferation, and the lactobacillus plantarum 589 also has a low dosage concentration of only 1x10<7> CFU for promoting splenic lymphocyte proliferation.

Description

[0001] The invention relates to the technical field of biological food, in particular to an exopolysaccharide produced by Lactobacillus plantarum, a preparation method, application and a composition containing Lactobacillus planta or exopolysaccharide. Background technique [0002] The exopolysaccharides produced by lactic acid bacteria are mucus or capsular polysaccharides secreted outside the cells during their growth and metabolism. There are many lactic acid bacteria producing exopolysaccharides in nature, which are mainly isolated from traditional fermented foods (such as dairy products, kimchi, sourdough, etc.). There are many factors that affect the synthesis of exopolysaccharides of lactic acid bacteria. The ability of different species of lactic acid bacteria to produce exopolysaccharides is obviously different. 350mg / L, the exopolysaccharide production of L.plantarum is 30-200mg / L, the exopolysaccharide production of L.delbrueckiisubsp.bulgaricus is 60-150mg / L, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/20A61K31/715A61K35/747A61P37/04C12R1/25
CPCC12P19/04C12N1/20A61K31/715A61K35/747A61P37/04
Inventor 孙盛陈作国陈苏俞赟霞朱珺李言郡余腾斐陈丽娥陈琳周晴晴陈彩玲郑志瑶任学良李宝磊李理
Owner HANGZHOU WAHAHA TECH
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