A strain of Lactobacillus paracasei with the function of lowering triglyceride and its application
A triglyceride and paracheese technology, applied in the field of microorganisms, can solve the problems of low triglyceride degradation rate, sleep problems, side effects, etc., and achieve the effects of excellent resistance to the adverse environment of the digestive tract and high safety.
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Embodiment 1
[0035] Example 1 Screening of Lactobacillus paracasei (Lactobacillus paracasei) HCS17-040CGMCC No.19747 bacterial strain
[0036] 1. Primary screening
[0037] Add 500uL healthy male baby feces samples to 4.5mL modified MRS (pH to 5.5) liquid medium for dilution, and dilute to 10 by 10-fold dilution method -6 , from 10 -3 -10 -6 Take 50uL of the sample solution for the dilution and spread it on the improved MRS solid medium plate, two parallels for each gradient, put the plate in a ziplock bag and culture it normally at 37°C for 24-48 hours;
[0038] 2. Purification
[0039] Pick a single colony with typical characteristics of the target strain (observation shape, size, color, and transparency, etc.), large colony, and strong activity, and carry out streaking and purification culture on the improved MRS solid medium plate, and culture at 37 ° C for 24 ~ 48h, so repeated 2 to 3 times, until the characteristics of the colonies in the streaked plate are consistent;
[0040] ...
Embodiment 2
[0041] Identification of embodiment 2 Lactobacillus paracasei (Lactobacillus paracasei) HCS17-040 bacterial strain
[0042] The pure culture separated and purified in Example 1 was further determined to be a pure culture through streaking and smear microscopy, and then the strain identification was carried out, including Gram staining test, contact enzyme test and 16S rDNA full sequence sequencing Identification. The final identified and isolated strain was a strain of Lactobacillus paracasei, named Lactobacillus paracasei (Lactobacillus paracasei) HCS17-0140, which was preserved in the "Common Microbiology Center of China Committee for the Collection of Microbial Cultures" on April 27, 2020. The deposit number is CGMCC No.19747.
[0043] The above-mentioned Lactobacillus paracasei HCS17-040 strain is Gram-positive, with white colonies, round protrusions, smooth and dense surface, neat edges, and cells in the shape of elongated rods, arranged singly or in pairs or in chains. ...
Embodiment 3
[0044] Embodiment 3 Lactobacillus paracasei (Lactobacillus paracasei) HCS17-040CGMCC No.19747 bacterial strain is resistant to digestive tract adverse environment test
[0045] The ability of Lactobacillus paracasei (Lactobacillus paracasei) HCS17-040 to withstand the adverse environment of the digestive tract is a prerequisite for its ability to reach the intestinal tract with a high survival rate, survive in the intestinal tract, colonize and grow, and exert its efficacy.
[0046] 1. Acid resistance test
[0047] The three-passage bacterial solution was respectively inoculated into the blank control medium and the basic MRS medium of pH 3.0 according to the inoculum amount of 10%. Static culture at 37°C, sampling at 17 hours, 10-fold serial dilution with sterilized normal saline, respectively take 1000 μL of bacterial solution with appropriate dilution for mixed bacteria counting operation, repeat twice for each dilution, and static culture at 37°C Count after 36-48 hours.
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