Application of a strain of Lactobacillus fermentum in the preparation of food or medicine with the function of lowering triglyceride
A technology for fermenting lactobacillus and triglycerides, applied in the field of microorganisms, can solve problems such as low triglyceride degradation rate, memory or mental problems, sleep problems, etc., to improve host immunity, good bacteriostatic properties, and prevent bacterial effect of diarrhea
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1 Screening and Physiological and Biochemical Characteristics of Lactobacillus fermentum HCS08-005 CGMCC No.16259 Strain
[0028] Add 5 g of baby feces to 5 mL of buffered peptone solution, and dilute to 10 by 10-fold dilution method -3 , each dilution was streaked onto MRS modified medium plates. Cultivate at 37°C for 24-48 hours (put the plate into a ziplock bag); pick a single colony with typical characteristics of the target strain (observation of shape, size, color, and transparency, etc.), larger colonies, and stronger activity, and use it in the MRS-improved The culture medium was streaked and purified, and this was repeated 2 to 3 times until the characteristics of the colonies in the streaked plate were consistent; two or more single colonies were picked from each purified plate for smearing, Gram staining, and observation under the microscope. Observe whether the color and the shape of the bacteria are consistent, so as to determine whether the coloni...
Embodiment 2
[0030] Example 2 Identification of Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005 CGMCC No.16259 strain
[0031] The pure culture separated and purified in Example 1 was further determined to be a pure culture by streaking and smear microscopy, and then carried out bacterial species identification, including Gram staining test, contact enzyme test, sugar fermentation identification, API Identification, and 16S rDNA full sequence sequencing identification. The final identification and isolation of the isolated strain was a strain of Lactobacillus fermentum, named Lactobacillus fermentum HCS08-005, which was deposited in the "General Microbiology Center of China Microbiological Culture Collection Management Committee" on August 14, 2018. The number is CGMCC No.16259.
[0032] The above-mentioned Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005 strain is a Gram-positive bacterium, facultatively anaerobic, without spores, and immobile; the colonies are sma...
Embodiment 3
[0033] Example 3 Resistance to digestive tract adverse environment test of Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005 CGMCC No.16259 strain
[0034] The ability of Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005 to withstand the adverse environment of the digestive tract is a prerequisite for it to reach the intestinal tract with a high survival rate, survive in the intestinal tract, colonize and grow, and exert its efficacy.
[0035] 1. Acid resistance test
[0036] The three-passage bacterial solution was inoculated into blank control, MRS medium with pH 2.0 and pH 3.0 respectively according to the inoculum amount of 10%. Static culture at 37°C, sampling at 17 hours, 10-fold serial dilution with sterilized physiological saline, respectively take 1000 μL of appropriate dilutions for counting viable bacteria, repeat twice for each dilution, and static culture at 37°C Count after 36-48 hours.
[0037] Acid resistance test data indicators:
[0038...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More