Application of Lactobacillus fermentum in preparation of food or medicine with function of reducing triglyceride
A technology for fermenting Lactobacillus and triglycerides, applied in the field of microorganisms, can solve problems such as low triglyceride degradation rate, memory or mental problems, sleep problems, etc., to improve host immunity, good antibacterial properties, and prevent bacterial infection The effect of diarrhea
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Embodiment 1
[0027] Example 1 Screening and Physiological and Biochemical Characteristics of Lactobacillus fermentum HCS08-005 CGMCC No.16259 Strain
[0028] Add 5 g of baby feces to 5 mL of buffered peptone solution, and dilute to 10 by 10-fold dilution method -3 , each dilution was streaked onto MRS modified medium plates. Cultivate at 37°C for 24-48 hours (put the plate into a ziplock bag); pick a single colony with typical characteristics of the target strain (observation of shape, size, color, and transparency, etc.), larger colonies, and stronger activity, and use it in the MRS-improved The culture medium was streaked and purified, and this was repeated 2 to 3 times until the characteristics of the colonies in the streaked plate were consistent; two or more single colonies were picked from each purified plate for smearing, Gram staining, and observation under the microscope. Observe whether the color and the shape of the bacteria are consistent, so as to determine whether the coloni...
Embodiment 2
[0030] Example 2 Identification of Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005 CGMCC No.16259 strain
[0031] The pure culture separated and purified in Example 1 was further determined to be a pure culture by streaking and smear microscopy, and then carried out bacterial species identification, including Gram staining test, contact enzyme test, sugar fermentation identification, API Identification, and 16S rDNA full sequence sequencing identification. The strain finally identified and isolated was a strain of Lactobacillus fermentum, named Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005. The number is CGMCC No.16259.
[0032] The above-mentioned Lactobacillus fermentum HCS08-005 strain is Gram-positive, facultatively anaerobic, non-spore-free, and immobile; the colonies are small and uniform in size, with neat edges, smooth and compact surface, white or milky white; It is short rod-shaped, arranged in piles or chains; its physiological and bioche...
Embodiment 3
[0033] Example 3 Resistance to digestive tract adverse environment test of Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005 CGMCC No.16259 strain
[0034] The ability of Lactobacillus fermentum (Lactobacillus fermentum) HCS08-005 to withstand the adverse environment of the digestive tract is a prerequisite for it to reach the intestinal tract with a high survival rate, survive in the intestinal tract, colonize and grow, and exert its efficacy.
[0035] 1. Acid resistance test
[0036] The three-passage bacterial solution was inoculated into blank control, MRS medium with pH 2.0 and pH 3.0 respectively according to the inoculum amount of 10%. Cultivate statically at 37°C, take samples at 17 hours, perform 10-fold serial dilution with sterilized normal saline, take 1000 μL of appropriate dilutions of bacteria solution for counting viable bacteria, repeat twice for each dilution, and culture statically at 37°C Count after 36-48 hours.
[0037] Acid resistance test da...
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