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Improved culture method of swine-derived lactobacillus reuteri

A technology of Lactobacillus reuteri and its cultivation method, applied in the field of improved cultivation of Lactobacillus reuteri from pigs, can solve the problems of lack of systematic research

Pending Publication Date: 2020-12-01
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Lactobacillus reuteri from porcine has a good application prospect in pig production, but there is a lack of systematic research on its in vitro culture conditions

Method used

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  • Improved culture method of swine-derived lactobacillus reuteri
  • Improved culture method of swine-derived lactobacillus reuteri
  • Improved culture method of swine-derived lactobacillus reuteri

Examples

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Effect test

Embodiment 1

[0026] Example 1 Screening pig source Lactobacillus reuteri optimal culture conditions

[0027] (1) MRS liquid medium was prepared, sterilized by high pressure and cooled to room temperature for later use.

[0028] (2) Inoculate Lactobacillus reuteri from porcine (isolated from pig intestinal contents and stored in China Industrial Microorganism Culture Collection Center, strain number: CICC 6118) into MRS medium and culture them for 12h and 24h respectively , 36h and 48h, and other culture conditions were the same (temperature 37°C, inoculum size 5%, rotation speed 120r / min, anaerobic), and then use a spectrophotometer to detect the OD600 absorbance value of the bacterial solution for different culture periods. The measurement results showed that the optimal culture time of Lactobacillus reuteri from porcine was 36h ( figure 1 ).

[0029] (3) Lactobacillus reuteri from porcine was inoculated into MRS medium, cultured at 35°C, 37°C, 39°C, 41°C and 43°C respectively, other cu...

Embodiment 2

[0034] The improved culture method of embodiment 2 pig source Lactobacillus reuteri

[0035] Lactobacillus reuteri from porcine was inoculated into MRS medium for cultivation. The culture conditions were as follows: the inoculation amount of Lactobacillus reuteri from porcine was 3% of the volume of the medium, the culture temperature was 41°C, the rotation speed was 60r / min, anaerobic And supply carbon dioxide (the content of carbon dioxide in the incubator is about 20%), cultivate 36h ( Figure 6 ).

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Abstract

The invention discloses an improved culture method of swine-derived lactobacillus reuteri. The method comprises the following steps: inoculating swine-derived lactobacillus reuteri into an MRS culturemedium, and performing culturing for 36-48h at 37-43 DEG C at a rotating speed of 0-120r / min in the absence of oxygen and with carbon dioxide. According to the invention, the culture conditions of the swine-derived lactobacillus reuteri are optimized and improved; and according to the invention, a plurality of environmental conditions such as culture time, temperature, oxygen content, rotating speed and inoculum size are optimized and improved, and the formulated culture method can be used for efficiently culturing the swine-derived lactobacillus reuteri in vitro.

Description

technical field [0001] The invention relates to microorganism culture technology, and specifically describes an improved culture method for pig-derived Lactobacillus reuteri. Background technique [0002] Gut microbes specifically refer to the microbial community living and active in the intestinal tract of the host, which has many biological function genes that the host itself does not have. Intestinal microbes play an important role in host nutrient digestion and absorption, body immunity, and disease occurrence. In recent years, with the development of microbial research, a large number of probiotics, including lactic acid bacteria, bifidobacteria, and yeasts, have been discovered in animals, and are widely used in disease treatment, nutritional improvement, and food addition. The in vitro expansion culture of probiotics is the basis for their production and application. Therefore, exploring the in vitro culture methods of probiotics is of great significance for the deve...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20
Inventor 王海飞周雅静包文斌齐晓艺顾学珠吴圣龙
Owner YANGZHOU UNIV
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