Method for producing core seeds of rice two-line photothermosensitive male sterile line

Abstract

The invention discloses a method for producing core seeds of two-line photo-temperature-sensitive male sterile lines of rice. The production method includes 8 steps of sowing in advance, partial transplanting, plant selection, cold water treatment, plant trimming, fertility testing, cutting and transplanting, and multiplication. In the present invention, the sterile line plants with relatively consistent development process are selected, and after being treated with cold water at 23.5°C±0.1°C for 6 days, the stems and ears in the state of "leaf pillow flat" at the end of the 6th stage of booting are selected for microscopic examination of fertility, and larger groups are operated , and control the elimination rate to prevent "drift" while ensuring that the species remains unchanged; using cutting backs to regenerate seeds and Hainan winter breeding production, coupled with effective isolation methods, can quickly obtain enough seeds in two planting seasons in different places a year. Quantity of core seeds of rice two-line photothermosensitive male sterile line.

Description

technical field [0001] The invention relates to a method for producing core seeds of a two-line photothermosensitive male sterile line of rice. Background technique [0002] Compared with three-line hybrid rice, two-line hybrid rice has the advantages of free grouping and concise procedures, and it is easier to select and breed new rice varieties with excellent traits. Two-line hybrid rice has become the mainstream of hybrid rice production, and it plays a very important role in production. The large-scale popularization of two-line hybrid rice benefits from the successful application of two-line photothermo-sensitive male sterile lines in rice. Cross-breeding, the males are fertile in short-day low-temperature environment, and are used to reproduce the sterile line itself. [0003] The two-line CMS widely used in rice is of the light-temperature interaction type, that is, the day length factor and the environmental temperature factor cooperate to control the fertility exp...

AI Technical Summary

Problems solved by technology

Previously, the selected CMS plants were separated and tested, and the performance of the hybrid offspring was observed to ensure that the matching characteristics did not change. The operation was cumbersome, time-consuming and laborious, and it was difficult to apply.