Design method of DNA nanoprobe structure with efficient conversion rate
A nanoprobe and design method technology, applied in the field of DNA nanoprobes, can solve the problems of conversion efficiency space restriction and enzyme activity limitation, and achieve the effects of reducing errors, enhancing detection signals, and improving conversion efficiency
Pending Publication Date: 2020-12-04
CHONGQING TECH & BUSINESS UNIV
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] In the previous construction of the signal conversion strategy, in order to change the traditional detection mode of the probe:target:signal ratio of 1:1:1, a signal conversion and enzyme amplification strategy was designed. However, the conversion efficiency in this method is limited by the space restriction and enzymatic activity, further in order to avoid these problems, we designed a high-efficiency conversion rate DNA nanoprobe structure design method
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0025] see figure 1 , a high-efficiency conversion rate DNA nanoprobe structure design method, which introduces the palindrome structure into the Holliday junction structure, constructs an enzyme-free and efficient signal conversion system, and increases its conversion efficiency to 1:3, including the following step:
[0026] S1, carry out the design of single chain palindrome sequence, please refer to figure 2 , the sequence read from 5' to 3' on the single strand of the palindromic sequence is consistent with the sequence read from the same 5' to 3' on the complementary strand, there is a symmetry center, and the bases on both sides of the symmetry center are symmetrical about the symmetry center , can form complementarity, select the type of base, and optimize the number of bases. When designing, because the palindromic sequence is easy to form a hairpin structure, but the palindromic sequence is applied to the DNA nanoprobe and further When used for signal conversion, t...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a design method of a DNA nanoprobe structure with efficient conversion rate, and belongs to the technical field of DNA nanoprobes. A palindrome structure is introduced into anHoldi connection structure, an enzyme-free high-efficiency signal conversion system is constructed, the conversion efficiency is increased to 1: 3, and the method comprises the following steps: S1, designing a single-chain palindrome sequence; and S2, on the basis of the principle of the Heldi connection structure, investigating the synthesis yield and stability of the structure through theoretical calculation and contrast experiments, and designing four DNA single chains A1, S1, S2 and S3; According to the design method of the DNA nanoprobe structure with efficient conversion rate, firstly, the DNA body sequence has encodability, so that the method can be universally suitable for various target analytes by designing corresponding DNA capture sequences according to different targets; and secondly, the input of a target object of one molecule can be converted into the output of three DNA single chains, and a detection signal can be enhanced on a molecular conversion level.
Description
technical field [0001] The invention belongs to the technical field of DNA nano-probes, in particular to a design method for a high-efficiency conversion rate DNA nano-probe structure. Background technique [0002] In recent years, signal conversion has gradually become a new detection mode in analysis and detection. This assay, which converts target detection into substances that are easier to capture and identify, is expected to improve both the sensitivity and accuracy of detection. Therefore, the detection method of signal conversion mode has become a research hotspot in recent years. For example, in the quantitative analysis of proteases, researchers select a specific catalytic substrate, and under the catalysis of the target protease, convert the substrate into an electrochemically active catalytic product, and quantify the target by directly measuring the electrochemical signal of the catalytic product. Protease, thereby realizing the determination of protease by el...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/682C12Q1/6834
CPCC12Q1/682C12Q1/6834C12Q2525/205C12Q2525/301C12Q2565/125C12Q2563/143C12Q2563/149
Inventor 杨哲涵卿敏吴虹张晓龙
Owner CHONGQING TECH & BUSINESS UNIV