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Method for counting phytoplankton in seawater on site

A phytoplankton, counting method technology, applied in computing, data processing applications, office automation and other directions, can solve problems such as low efficiency, semi-automatic counting technology cannot be applied on-site, and achieve high efficiency, easy to popularize, easy to operate and easy to learn. Effect

Active Publication Date: 2020-12-04
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even so, there is still the problem of low efficiency
[0012] In addition, and most importantly, none of the existing automated / semi-automated counting technologies can achieve field application
[0013] To date, survey methods that can directly and accurately determine the number of phytoplankton in seawater samples in the field are still urgently established

Method used

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  • Method for counting phytoplankton in seawater on site
  • Method for counting phytoplankton in seawater on site
  • Method for counting phytoplankton in seawater on site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the rapid counting of Prorocentrum micronum in seawater

[0037] Prorocentrum miniature is also known as Prorocentrum miniature. The shell surface of the algae is heart-shaped or oval, with a body length of 15-23 μm, a width of 13-17 μm, and a top thorn of about 1 μm. It is the main red tide in the temperate and subtropical regions of the world. one of the causative organisms. The invention realizes the rapid counting of the algae in the seawater, and uses the classic microscopy method to carry out comparative verification.

[0038] Step 1, such as figure 1 As shown, the C&B 8-A portable 8-channel C&BMS mainframe (4) produced by Qingdao Gufeng Experimental Instrument Co., Ltd. and the ThinkBook 14 portable notebook computer (6) produced by Lenovo were properly placed on the survey ship. Connect the C&BMS host (4) and the laptop (6) with a data cable (7). Turn on the machine, click the special software TER-GO, and set the parameters for measuring the σ v...

Embodiment 2

[0047] Embodiment 2: The concentration optimization experiment of SDS dispersion liquid

[0048] The seawater information used in the experiment is shown in Table 1:

[0049] Table 1 Information of seawater samples used in experiments

[0050]

[0051] The steps are the same as Step 1 of Example 1.

[0052] Step 2: Take 3L of S1 seawater with a columnar water collector (model 3L, product of Qingdao Shanglong Xinda Trading Co., Ltd.), and transfer it to a clean gallon barrel.

[0053] Step 3: Use a 200 μm sieve (product of Yantai Heqi Experimental Instrument Co., Ltd.) to filter seawater to prepare a pretreated seawater sample S1P1.

[0054] Step 4: Put 10mL of pretreated seawater sample S1P1 into twelve 50mL centrifuge tubes, and then add 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11% and 12% SDS dispersions, shake and mix to make mixed samples S1H1, S1H2, S1H3, S1H4, S1H5, S1H6, S1H7, S1H8, S1H9, S1H10, S1H11 and S1H12 .

[0055] Step 5 is the same as Step 4 in Example ...

Embodiment 3

[0067] Example 3: On-site counting of phytoplankton in seawater near the coast of Qingdao

[0068] Sampling site: 120°21.00′E; 35°57.50′N

[0069] Sampling water depth: 0.5m

[0070] Sampling time: August 31, 2020

[0071] Survey vessel: Zhongyuke No. 101

[0072] The steps are the same as Step 1 of Example 1.

[0073] Step two and step three are the same as step two and step three of embodiment 2.

[0074] Step 4: Put 10 mL of the pretreated seawater sample S1P1 into a 50 mL centrifuge tube, then add 5% SDS dispersion liquid into the centrifuge tube, oscillate and mix to prepare the mixed sample S1H1.

[0075] Step five and step six are the same as step five and step six of embodiment 2.

[0076] Step 7, start the special software of C&BMS (4) on the laptop (6), record the capacitively coupled non-contact conductance σ value of the mixed sample S1H1 in each channel in real time, and obtain 8 curves as follows ( figure 2 ):

[0077] Step 8: The record graph of C&BMS sh...

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Abstract

The invention relates to a method for counting phytoplankton in seawater on site and belongs to the field of marine survey methods. The method comprises the following steps of: collecting a target water sample, and making the target water sample pass through 200 mu m bolting silk to prepare a seawater sample P1; oscillating and uniformly mixing the seawater sample P1 and sodium dodecyl sulfate solution of the same volume in a centrifugal tube to prepare a mixed sample H2, injecting the mixed sample H2 into eight long-neck glass funnels on a chemical and biological process online monitoring system respectively at the same time, and enabling the solution to flow into a container below through the funnels under the action of gravity; automatically acquiring / recording capacitive coupling non-contact conductance sigma data of the flowing mixed sample H2 by adopting a chemical and biological process online monitoring system; after making all the mixed sample H2 completely flow out of the long-neck funnels, enabing the chemical and biological process online monitoring system to automatically count the number n1, n2,..., n8 of inverted peak type signals appearing in each detection channel.The method can measure the number of phytoplankton in the seawater sample on site, and is simple and rapid.

Description

Technical field: [0001] The invention belongs to the field of marine survey methods, in particular to an on-site counting method for phytoplankton in seawater. Background technique: [0002] Phytoplankton refers to tiny plant groups that live in plankton in water bodies. It is the most basic and important link in the food chain of aquatic ecosystems, and it is one of the important indicators for monitoring, evaluating and risk warning of water quality and eutrophication. Accurately obtaining the number of phytoplankton taxa in the marine environment is one of the main purposes of marine surveys. [0003] The current methods for obtaining the number of phytoplankton groups in seawater basically include the following steps: collection, fixation, transfer, concentration and counting. [0004] Microscopy is the most classic counting method, but this method takes a long time and requires high professional knowledge. The result is easily disturbed by non-algae particles in water ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/02G01N27/06G06Q10/10
CPCG01N15/02G01N27/06G06Q10/101
Inventor 陈聚法曲克明赵俊张旭志江涛
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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